Expressions Of RAGE, FAS And ENOS In Heart, Brain, Artery And Lung Of Macaques With The Spontaneous Type â…¡ Diabetes

This study was sought to investigate the histopathology structural changes of the heart, brain, arteries and lung in the spontaneous type2diabetes macaques (Western Sichuan subspecies). We Explored the expression of receptor for advanced glycation end products (RAGE), fatty acid synthase (FAS) and endothelial nitric oxide synthase (eNOS) protein in the heart, brain, arteries and lung with spontaneous type2diabetes macaques, and aimed at further understanding the change rules and pathology significance in the expression and localization of these factors in the spontaneous type2diabetes macaques model. In present study we analyzed the structural changes of macaques’ heart, brain, arteries and lungs by HE staining, and evaluate the expression profile of RAGE, FAS and eNOS using immunohistochemistry (IHC) assay. The results are as follows:(1) Histopathological changes in heart, brain, artery and lung:In Diabetes Mellitus group, we noticed alveolar atrophied, alveolar septa widened, congested in lung and pulmonary interstitial collagen and elastin increased. No significant pathological change was observed in other organs.(2) Expression and Distribution of RAGE, FAS and eNOS in Diabetes Mellitus macaques:RAGE positive products mainly distributed in myocardical cell nuclei, thalamus and cerebral cortex neurons, cerebellar granular layer neurons, while in the artery the RAGE they were mainly distributed in intima, media and adventitia. RAGE also distributed in type Ⅰ alveolar cells in lung. According to the distribution and expression of RAGE in different organs, compared with the healthy group, there was significant difference about the mean optical density of thalamus, brain, cerebellum and artery in diabetes group (P<0.05), there was highly significant difference about the mean optical density of heart and lungs in diabetes group compared with healthy group (P<0.01). The result of positive area in diabetes group were identical to the result of average optical density in diabetes group except for the lung, there was highly significant difference about the positive area of lung in diabetes group compared with the health group.FAS positive products mainly distributed in myocardial cell nuclei. While in the brain, FAS mainly distributed in thalamus and cerebral cortex neurons and cerebellar Purkinje cells; FAS also distributed in the artery and the lung alveolar type Ⅱ cells. According to the distribution and expression of FAS in different organs, it is obviously that:there was significant difference about the mean optical density of the cerebellum and artery in diabetes group compared with the healthy group (P<0.05), while there was highly significant difference about the heart, thalamus, cerebrum and lungs in diabetes group compared with the healthy group (P<0.01). The result of positive area in diabetes group was consistent with the result of mean optical density in diabetes group.The distribution and expression of eNOS positive products were in accordance with the result of RAGE. Compared with the control group, there was significant difference about the mean optical density of the heart, cerebellum and artery in diabetes group compared with the control group (P<0.05), while highly significant difference was noticed in thalamus, cerebrum and lungs (P<0.01). The result of positive area about the RAGE positive products in diabetes group was consistent with the result of mean optical density in diabetes group.The RAGE, FAS and eNOS were all expressed in the heart, brain, arteries and lungs, and all have significant upregulation in diabetes group. According above research we can concluded that these factors may be involved in the complications of diabetes in macaques.