The Dynamic Expression Of TLR9 And Relative Inflammatory Factors In Brain Rats And Intervention

Objective:To investigate the effect of TLR9 and the related inflammatory factors and the mediation mechanism of brain injury, immunohistochemical ultra sensitive SP method has been used to examine the dynamic expression of TLR9 and the related factors in the normal rats, injected with E. coli and Coptis Decoction prevention rats in the present study.Methods: 66 rats were randomly divided into three groups. Normal control group (n = 6): synchronize feeding with other groups, dealing with the day of intraperitoneal injection of saline. Test group (n = 30): simultaneous feeding with other groups to deal with the day of intraperitoneal injection of E. coli were 0.5 mL(2.4×109 / mL), n = 5. Coptis Decoction prevention group (n = 30): synchronous breeding with other groups, each according to 12.6mL / kg orally in advance Coptis Decoction 6 days, dealing with the day of intraperitoneal injection of E. coli were 0.5 mL(2.4×109 / mL), n = 5. The rats of each control group and experimental group and prevention group were killed randomly at 3, 6, 9, 12, 18, 24 hours after infection of E. coli. The expression of TLR9 and the related factors in brain were analyzed by immunohistochemistry.Result:1. The expression of TLR9TLR9 in the body under normal circumstances, a small amount of expression. When the injection of E. coli, TLR9 expression showed a significant change, and there are significant differences in characteristics such as the more obvious over time. The time changes in the amount of TLR9: TLR9 during 3h after infection as compared to normal physiological conditions, increased significantly, beginning at 6h, a significant increase, 12h and reached its peak with the control group were significantly different. Prevention group were significantly lower than the attack TLR9 drug group, the difference was significant. Prevention and control groups were not significantly different2. The expression of IL-4Each time the control group had weak expression. The expression of the treatment group were significantly higher expressed in the control group. The expression of the time they are different, 3h expression increased to 9h, 12h to 24h at a higher level, of which the highest 18h, 24h decreased slightly. Poison attack, compared with the control group, in 3h, 6h significant difference, 12h significantly different, but the overall comparison, no statistical significance. Coptis Decoction prevention group compared with the attack are significantly reduced.3. The expression of IL-6, IL-12, IFN-γIL-6, IL-12, IFN-γ, control group the expression of all time are weak,the treatment group and Coptis Decoction prevention group have clear expression. The expression of treatment group was significantly higher than the control group and prevention group, there was a significant difference. Coptis Decoction prevention group compared with the control group, no significant difference.The results show that, Coptis Decoction could significantly inhibit the E. coli-induced TLR9, IL-6, IL-12, IFN-γrelease, and the time-effect relationship was obvious.