Synthetic Oviductal Fluid Added To The Serum Of Cattle In Vitro Fertilization And Embryo Development Rate

College ofAnimal and Veterinary Sciences, Southwest Agricultural University~ ChongKing.400 716. Abstract In order to study the effect of chemically defined and undefined medium on the development of yellow cattle embryo, Here we have investigated the rates of the oocytes maturation, fertilization and formation of the blastocyst with the standard procedures for in vitro embryo production, the experiment was carried out in three different stages. (i) After 22h of culture in the TCM 199 containing 0.1 p,g/ml E2, 30~tgIml FSH and 10%FCS, 73 of 80 oocytes were found to have excluded the first polar body under the phase-contrast microscope by tabletting and staining. (ii) Sperm penetration rate was 71 of 80 oocytes (88.8%) after 12h of insemination in the BO medium containing 5IUIml heparin, 10mM caffeine and 20mg/mi BSA, single-sperm rate was 87.3%(62/80) and poly-sperm rate was 12.7%(9180). (iii) To compare with the effect between the defined and undefined medium on bovine embryo development, 5%FCS was added to the SOFaa in the test group and was not in the control group. There抯 no significant difference of rate of cleavage between the test group( 72 % , 18/25 in SOFaa-FCS) and the control(82.4% , 14/l7in SOFaa). The rate of formation of blastocytes in SOFaa containing FCS(40% ,10/25) was higher than that in SOFaa (11.8%, 2/17 )(P<0.05). It is reasonable, therefore, to conclude that when FCS was added to the SOFaa, it can promote distinguishly the blastocyst development. SOFaa, which is a chemically defined medium, lacked some effective components that were essential to the development of yellow cattle embryo after cleavage, much work remains to be done with it.