| The purpose of this article is to strengthen the preservation of Yanbian yello w cattle breeds, to maintain species diversity and ecological balance, to develop a nd continual use of Yanbian yellow cattle breeds. This study is focused on how t o improve the pregnancy rate of external fertilized embryo transfer of Yanbian y ellow cattle. This experiment has been carried out by different culture methods of external fertilized embryo in each stage, operating technology and the quality of blastocysts. The results showed that.1. This experiment performed around how to improve the pregnancy rate, to reduce production cost and to simplify the operating procedure of external embr yo production system in Yanbian yellow cattle. The results showed that the clea vage rate of cultured in 35mm dish(cheap) was significantly higher than that cult ured in 4 hole plate (expensive) in the same medium conditions (P<0.05) but the re was no difference in blastocyst rate; no difference between PBS liquid sperm treatment group and BO liquid sperm treatment group in cleavage rate and blasto cyst rate. PBS group was more simple and cheaper than BO group, comparative st udy of fertilization time, PBS 22h group has significant difference than PBS grou p 6h,BO 6h and 22h group (P＜0.05).2. Improving the quality of blastocysts and increasing the quantity of eel Is are important. Blastocysts full of cells are good for single blastocys analysis. Normally several blastocysts are required to for DNA analysis, whereas single blastocysts are required if they contain increased number of cells. Blastocyst tra nsfer is often used in bovine transplantation. Hence, blastocysts with high qualit y morphology and with a number of cells may improve the pregnancy rate afte r transplantation. Embryonic stem (ES) cells derived from the inner cell mass (I CM) of can be established from mice and humans. Attempts to establish ES eel 1 lines from livestock failed, partly due to the unsuitable culture conditions. The refore, developing new FVC systems to improve ES cell lines are successfully e stablished.This investigation compared the effect of human-induced pluripotent stem c ell (hiPS) medium with in vitro culture (IVC)-Ⅱ medium on bovine in vitro fert ilized early blastocysts. After 2 days culturing, the diameter of the blastocyst si gnificantly different from bovine blastocysts cultured in hiPS medium and those cultured in IVC-Ⅱ medium (150.30±29.49 μm vs. 195.58±41.59 μm, P<0.01). Furthermore, the total number of blastocysts cells cultured in hiPS was double of those cultured in B-IVC-Ⅱ medium (172.12±45.08 vs. 604.83±242.64, P<0.0 1). These results indicated that hiPS medium significantly improve the quality o f bovine blastocysts.3. Obtained the local characteristics of Yanbian yellow cattle breeds by external fe rtilization for the first time. The subjects of this experiment were local Yanbian Yellow Cattle.It improved the efficiency of beef cattle breeding by embryo transfer tec hnology, increased the rate of cow calving rate. Improvement and promotion of Estru s synchronization, embryo transfer and other biotechnology, will accelerate the larg e-scale breeding process and play a key role in the protection and propagation of loca 1 varieties.The experimental results showed that there were no significant difference in estrus synchronization group1(supplemented with Vitamin A,D,E), group 1 and c ontrol group(P<0.05), the highest pregnancy rate group was group 1, estrus synch ronization of 43, 8 became pregnant as detected after embryo transfer 27, and g ave birth to 7 calves. Pregnancy rate and calving rate were respectively 29.63% and 16.28%. The lowest group of pregnancy rate was control group, embryos re constructed were introduced into 19 surrogate mothers, 4 became pregnant as de tected after embryo transfer, and gave birth to 2 calves(twins). Pregnancy rate a nd calving rate were 21.43% and 10.53%. The group 2, estrus synchronization of 49, 10 became pregnant as detected after embryo transfer 38, and gave birth t o 8 calves. Pregnancy rate and calving rate are 26.28% and 21.05%. |
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