Dalian Long Island Rose (rosa Rugosa Thunb.) Population Genetic Diversity And Genetic Structure Of A Preliminary Study

Wild Rosa rugosa thunb. has been ranked in the second protected plants and its germplasm, economic and ecological importance can't be ignored. But more and more disturbance from human beings lead to make the habitats of Wild Rosa rugosa fragmental and Wild Rosa rugosa distributed in the unsuccessively habitats. Corresponding to this, the genetic structure and diversity of Wild Rosa rugosa have been seriously influenced which put them into dangerous situation. In the fragmental habitats of Wild Rosa rugosa of Changshan island of Dalian city, 17 clusters(CSDl-CSD17) of Wild Rosa rugosa have been selected to be put in the experiment and their genetic structure and diversity in the fragmental habitats have been analyzed by RAPD. combined with fragmental traits and reproduction traits of Wild Rosa rugosa clusters, the experiment conclusion has been well interpreted and protection methods have been advanced.162 loci were amplified by RAPD, of which 148 loci (91.36%) were polymorphic and 4 loci were specific , 12 loci were monomorphic to some clusters. The average of P (percents of polymorphic loci) within clusters was 22.59%. The average of N_e (number of efficient allele) was 1.1387. The average of H (expected heterozygosity) within clusters was 0.0803.Shannon index is 0.1196. It's very low among clonal plants. The average of P (percents of polymorphic loci) within the population is 91.36%, H (expected heterozygosity) and / (Shannon index) are 0.2690 and 0.4412. There was great genetic variation in the population. Percent of polymorphic loci and Shannon index was positively correlative with the sample size and sample area ;Percent of polymorphic loci (F) and Shannon information index is positively correlative with the sample size. There are only one cluster have found clonal ones among 17 clusters.G_STGenetic divergence) of all RAPD loci among clusters was varied from 0.0141-1.0000.It's very different from all RAPD loci. The means of G_ST(Genetic divergence) was 0.6954.N_m (gene flow) was varied from 0.0000³4.9826.The means of N_m(gene flow) was 0.2190, which was greatly less than 1, so there was serious genetic drift within and among clusters.From Neis's unbiased measures of I (genetic identity) and D (genetic distance) by RAPD, the shortest genetic distance was between CSD4 and CSD5, which was 0.1006. The longest genetic distance was 0.3803 just between CSD3 and CSD16. Chi-square proof-test of all RAPD loci among clusters indicated that 80.25% of loci were prominently departure from Hardy-Winbergii equilibrium. Genetic identity was positively correlative with the space distance through Mental test.Compared with other populations of R. rugosa Thunb, R. rugosa Thunb in Changshan island of Dalian city have great genetic variation, but the habitat have been destroyed and degenerate in some area, which may result in the fragmentizing of the habit and the decrease of population genetic variation. The habitats should be restored to protect plants and habit in the coast and increase the population size to decrease the genetic differentiation among clusters, combined with manpower methodto hasten the exchange of gene flow.