Halophilic Archaea Bacteria Ab3 In Bacterial Rhodopsin And Phylogenetic Studies

Extreme halophiles, as a new kind of microorganism reasources, has offered many new questions for discussion in theory research, such as microbial physiology, heredity and taxology, life sciences and so on, and it has also offered new materials for life origin and evolution. The bacteriorhodopsin (BR) is the only protein found in the purple membrane of Halobacterium salinarum. It is very small and requires only light and a lipid bilayer to generate an electrochemical ion gradient, which can be used by the cell for ATP synthesis and other metabolic processes. Such unique photoelectric responses make BR the most promising biological material in many fields.Extreme halophiles are wide spread throughout the world, China also have abundant halophilic bacteria resource. In order to study and analyze the species and bacteriorhodopsin (BR) protein resource, a strain of halophilic archaea AB3 was isolated. Partial DNA fragments encoding for a BR protein and 16S rRNA encoding gene from AB3 were amplified by PCR, and their DNA sequences were determined. The results indicated that compared the deduced amino acid sequence with other existent BRs, BR protein from AB3 is visible different to others. The homology analysis about BR gene and 16S rDNA as well as phylognetic analysis about 16S rDNA show that the strain AB3 is a new member of the genus Natrinema. The GenBank accession number of the 16S rDNA sequences of strain AB3 is AY277583.