Diversity Of Halophilic Archaea Isolated From Differenthypersaline Lakes And Salt Fields

Hypersaline is rich in halophilic microorganisms resources, and Halophilic archaea have an important value in researching. In this paper, microorganisms were isolated from Gouchi, Huamachi and Lianyungang salt field. Then the studies had been conducted on archaea’s diversity after cultured. With 16 S rDNA sequencing and phylogenetic analysis, it showed that four strains isolated and purified from Gouchi belonging to the genus of Halorubrum; two strains isolated from Huamachi belonging to the genus Haloarcula and Halorubrum; ten strains isolated and purified from Lianyungang belonging to the genus Haloarcula and Halorubrum. Three regional populations showed similarities and uniqueness exsit in the distribution of archaea, which may be ambient temperature, salt content, pH and so on. 16 S rDNA gene sequencing technology and phylogenetic analysis are a classification determination of microbial species diversity of resources, which has limitation more or less. On the purpose of getting more information of detailed and accurate classification, we’d better conduct further taxonomic study.Using the method of non-culture techniques, further research had been conducted in Lianyungang on halophilic microbial diversity. Data of high-throughput sequencing showed a total of 6126 valid reads were obtained from the brine sampl. 535 operational taxonomic units(OTUs) were generated for archaea. All archaeal sequences fell into the Halobacteria class in Euryarchaeota phylum. When the genetic distance is 0.03, the samples’ Chao1, ACE and Shannon index were 764,834 and 4.76. The samples from Guannan solar saltern belong to forteen genera: Halorubrum(53.56%), Halonotius(22.22%), Halogranum(0.03%), Natronoarchaeum(0.11%), Halomicrobium(0.17%), Halolamina(0.17%), Haloarcula(2.32%), unclassified Halobacteriaceae(1.0%), Halobellus(4.86%), Haloplanus(5.32%), Halorhabdus(0.54%), Natronomonas(1.1%), no-rank-Halobacteriaceae(1.79%). What’s more, the dominate of genera are Halorubrum、Halonotius.Through traditional 16 S rDNA phylogenetic analysis, we found the dominant genu of Lianyungang Guanxi Salt fields were Halorubrum and Haloarcula, and high-throughput sequencing results showed Halorubrum, Halonotius were the dominant species. As well Halogranum, Natronoarchaeum, Haloarcula and other species had not been founded with 16 S rDNA phylogenetic analysis yet. 454 pyrosequencing is a more detailed description of the high-throughput sequencing for classification and identification of environmental microbiology, classification results.With pure culture and non-culture methods, combined with 16 S rDNA sequences and high-throughput sequencing methods showed that, Lianyungang, Shaanxi and other regions with abundant resources archaea, further in-depth study of community structure and diversity of composition, for China salt Lake, halophilic microbial diversity and phylogenetic analysis provides a basis theoretical research.