Traditional Polyphasic Taxonomic Identification Combined With Next-generation Sequencing Strategy Studying Resource And Evolution Of Extremophile

Microbes are the most widely distributed and the most in numbers and types, taking part in every cycle on the earth, from the interaction among different microbes to the globe carbon cycle. We concentrate on the new species isolation, resource utilization and species isolation and evolution analysis of archaea from salt-lake of Xinjiang Province.The abundant resource of marine provides us enough samples on studying drug reaserch, evolution analysis and resource utilization. The extreme environment is a excellent sample to study microbial origin and evolution, offering base for phyletic evolution.Archaean are also widely distributed, including lakes, oceans, hot springs and sediments. They play an import role in biological process. Besides, since many archaean live in extreme environments which are regarded as the early environments of the ecological earth, such as high temperature, anaerobic and so on. In addition, archaea are good models to study evolution.The archaean in this study were obtained using pure culture method, including some new species of different genus. We take advantage of some molecular markers to do evolution analysis.With the development of high-throughput sequencing and decreasement of sequencing cost. Microbial research come into a high-throughput area, not only in the traditional area. For instance,16S diversity research and meta-genome research.Different cultures were designed to improve the culture condition from diverse samples. Strain Maribacter thermophiles HT7-2T was utilized to do whole genome sequencing and construct genome annotation platform.Lastly, another suspected new archaea from salt lake was sequenced and comparative genomic analysis was conducted to study the evolution and find some genes related to environment adaptability.