Polyphasic Taxonomic Studies Of A New Marine Bacterium Gilvimarinus Polysaccharolyticus YN3~T And Genome Sequencing,Genomic Bioinformatic Analysis Of Six Halophilic Microorganism

The planet Earth formed from a molecular cloud by gravitational collapse in the old solar nebula contains gas, ice grains and dust about 4.6 billion years ago. After the long formation and evolution of the Earth’s ecosystem, there is now a colorful biosphere on it. The ocean occupies 71% surface of the Earth, and it is one of the most important ecosystems on the Earth. And microorganisms of ocean ecosystem are as the decomposers for mainly providing the elements to biogeochemical cycle. Some of them are autotrophic bacteria also can produce the organic materials and energy for the heterotrophic organisms. So far, the greater parts of marine microorganisms are still unknown and mysterious to human beings. Those microorganisms’biological characteristics, metabolic pathways, or environmental adaptation mechanism maybe have an important ecological value in the theory or industrial use.In this study, strain YN3T was isolated from the coastal algae samples was bulided as a new species of genus Gilvimarinus after phenotypic taxonomic study. On the basis of phenotypic, chemotaxonomic and genotypic data and DNA-DNA hybridization studies with the type strain Gilvimarinus chienesis and ’Gilvimarinus agarilyticus’, in this study we proposed that strain YN3T represents a novel species of the genus Gilvimarinus, for which the name Gilvimarinus polysaccharolyticus sp. nov. is proposed. After the polyphasic taxonomic studies of the stain YN3T, we extracted the genomic DNA for the whole genome sequencing using next-generation sequencing technology (Hiseq2000; Illumina) with paired-end libraries of 500 bp and 6,500 bp insert size. The genomic sequencing of strain YN3T resulted’19 contigs assembled into 7 scaffolds with the 3,746,057 bp length and 49.4%G+C content. And the whole genome sequence indicates the correspondence between its genomic characteristics and phenotypic features, and also provides a way to look for their environmental adaptability and survival mechanism.We also sequenced five more genomes of bacterial or archaeal strains. Halomonas zincidurans B6T was isolated from a deep-sea heavy metal rich sediment from the South Atlantic Mid-Ocean Ridge. Halomonas xinjiangensis TRM 0175T and Chromohalobacter israelensis DSM 6768 T are the phylogenetic closest two species of strain B6T. H. zincidurans B6T, H. xinjiangensis TRM 0175Tand C. israelensis DSM 6768 T may all have potential applications in the bioremediation of heavy metal contaminated environments. These data provides a new insight into further investigation of the heavy metal tolerance in moderately halophilic bacteria.Amphibacillus jilinensis strain Y1* is a tacultatively anaerobic and alkaliphilic bacterium isolated from a soda lake in China. We describe the structural and genetic features of the draft genome about the strain Y1T (3,831,075 bp, with a G+C content of 37.27%). The data will provide a new perspective of how microorganisms adapt to anoxic and alkaline environments, and may also provide a pool of functional enzymes which can work at higher pH.Natrinema altunense AJ2T is a strain of halophilic archaea isolated from a high-altitude (3884 m) salt lake in Xinjiang, China. This strain requires at least 1.7 M NaCl to grow, and can grow anaerobically in the presence of nitrate. We assembled 3,774,135 bp genomic sequence into 20 contigs (G+C content of 64.6%). Our detailed case study will contribute to our overall understanding about why Archaea can be found in such a diverse range of extreme environment.