With Low Calcium Sensitive Phenotype Of Arabidopsis Thaliana Natural Mutant Selection And Rough Mapping Of Related Gene

In order to understand the molecular mechanism of the plant growth in the low calcium situation, this experiment mainly makes the natural mutant Arabidopsis thaliana as material, filtrates out a kind of low calcium sensitive natural mutant Wt-5 through the filter of 96 kinds of natural variation types. By the phenotypic analysis, it is found that compared with Arabidopsis wild-type Col-0, Wt-5 has the phenotype of short roots and small and yellow leaves under the situation of the low calcium medium (contains 0μM CaCl2,20μM CaCl2), which almost has no differences with the phenotype in CK medium (contains lmM CaCl2).By atomic absorption spectrum method to measure the total calcium of Wt-5, it is found that compared with wild type Col-0, the total calcium content of Wt-5 grown in the CK is lower. Receiving low calcium treatment, Wt-5 still remains a lower content of the total calcium in the leaf (large leaf or small leaf).F1 and F2 plants can be got by hybriding Wt-5 and Ler(ecological), which has been shown to be the single recessive mutant genes by analysis. Using the map-based cloning, mutations can be roughly positioned within the scope of 18.14M-19.5Mbp of Arabidopsis chromosome 2.