Mechanisms Of CCDC-55 Regulating Calcium Nutrition In Arabidopsis

As a second messenger,Ca2+ responds to biotic and abiotic stresses,and is involved in many processes such as cell division,growth,transcriptional regulation,and so on.In addition,as a nutrient,Ca2+ is a component of the cell wall.CCDC-55 is the abbreviation of coiled-coil domain containing proteins 55,and the function of this protein is unknown.Phenotypic analysis of Ca2+ concentration gradient in Arabidopsis thaliana CCDC-55(AT2G27280)deletion mutant showed that the mutant had a low calcium sensitive phenotype.Calcium is a bivalent cation.It is unknown how ccdc-55 responds to four mineral elements,such as magnesium,iron,zinc,and manganese,which are required for other plants.Then the phenotypes of Mg2+,Fe2+,Zn2+ and Mn2+ concentration gradients were analyzed.It was found that compared with wild-type Arabidopsis thaliana,the mutant in culture medium with low iron and high zinc has obvious differences.However,the analysis results of element content in the mutant showed that there was no significant difference,which means that the gene is not involved in the storage of these three elements.The results of subcellular localization showed that CCDC-55 was localized in the nucleus.The results of promoter activity analysis showed that CCDC-55 was expressed in the young tissues of plants.It suggests that CCDC-55 may interact with other proteins in the cell to regulate some biological processes in plants.Moreover,Ca2+,Fe2+ and Zn2+ are indispensable in these processes.