The Expression Of Trehalase During Diapause In Artemia Sinica

Artemia sinica, also known as brine shrimp Artemia, which distributed worldwide in hypersaline water and over a wide temperature range.Artemia is widely distributed throughout the word, in China, mainly in the inland salt lake in Iner Momgolia, Xinjiang, Qinghai-Tibet Plateau, also appear in the Bohai bay and other coastal salt ponds. Artemia has strong anti ability to adapt to extreme enviroments such as low temperature and high salt,drying and other harsh environments. The peculiarity of Artemia made it widely used in the experiment.The main function of Trehalase is extracellular and intracellular hydrolysis into glucose to provide energy for biological activities of life. The synergistic effect of hormones regulate in vivo concentration of Trehalose and carbohydrates such as glucose, thereby effectively protect cells in vivo maladaptive environment outside. Trehalose can be synthesis through TPS/TPP, that is, uridine diphosphate glucose and glucose-6-phosphate synthesis trehalose-6-phosphate, helped along by Trehalase. Trehalose plays a important role while Artemia in diapause. Trehalase as the only capable of hydrolyzing Trehalose, has high research value. However,there has not been reported for the research of Trehalase in Artemia.In this study, the full-length cDNA of Trehalase was obtained and analyzed by bioinformatics and molecular evolution methods. The full-length of Trehalase is 2506 bp, containing 5’UTR and 3’UTR. The length ORF of Trehalase is 2111 bp, it encoded 703 amino acids, which contains a typical TreA domain. By multiple sequence alignment analysis shows that, Trehalase has been conserver structurally and functionally druing evolution. The expression pattern of Trehalase was investigated by quantitative Real-Time PCR. The results indicated Trehalase had a low expression during diapause. Along with Artemia development the expression quantity of Trehalse increased gradually. Temperature and salinity stress results that Trehalase has a low expression quantity during low temperature and high salt. While the external environment is suitable for Artemia, the expression quantity of Trehalse increased. The results of Real-Time PCR suggested that Trehalose would protect Artemia during diapause.The present study aims to clarify the Trehalase played an important role in diapause of Artmia. It is valuable to theory and application in research of diapause, having potential application value for developing and utilizing Artemia resource.