| Extremely low frequency electromagnetic fields (ELF-EMF) has been widely used in our daily life. Its exposure limit and its effect on human health has raised public concerns for a long time. But there is no consensus about the health effects induced by ELF-EMF.The effects of electromagnetic fields are caused by the exposure parameters of electromagnetic field, physical reactions, biological effects of chemical reactions and a series of joint-linking reaction. Observing the EMF exposure changes in real time at the organism to reveal the mechanism of electromagnetic fields on the health effects is very important.In this paper, to study the steady-state biological robustness of a life system being exposed to electromagnetic fields (EMFs), we measured the concentrations of reactive oxygen species (ROS) and intracellular calcium (Ca2+) in hippocampal neurons exposed to EMFs under various intensity, namely0,0.09,0.38,0.76,7.33or14.78mT, in an EMF exposure system in real-time, and analyzed the two parameters’steady points of states and steady domains before EMF disturbances, as well as their state transitions and robustness domains after the EMF exposure.Experimental results show that:(1) in the absence of EMF disturbance, the steady points are between46.157and120.913for ROS, and be-tween25.430and55.686for Ca2+, while the radius of stable domain is between1.688and10.278for ROS, and between2.782and13.345for Ca2+.(2) The ROS level is not affected by EMFs with intensity below7.33mT, but increased with step response when the EMF’s intensity is14.78mT; the Ca2+level is not affected by EMFs with intensity of0and0.09mT, but increased continuously with EMF disturbance of0.38mT.Some conclusions are drawn:(1) The states of intra-cellular Ca2+and ROS can keep stable in the absence of EMF disturbance;(2) the states can be changed by disturbances of stronger EMF, such as14.78mT;(3) the robust domain of hippocampal neurons is not related to the intensity of EMF disturbances. |
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