Phenotypic Identification The Ectopic Expression Of Different PtFT Genes Transcription And Signal Transfer After Grafting

Majority people agree that FT gene is the florigen in plants so far, FT protein can move in plants to promote plants flowering earlier. That provides a new way for fruit trees flowering regulation. I constructed Pt FT::GUS fusion expression vector based on precocious trifoliate orange FT gene cloned in our lab and convert it to the Poncirus trifoliata(L.) Raf. and Nicotiana tabacum. In the meantime, I am not only charactering transgenic tobaccoes phenotypic data of different Pt FT gene transcription but also finding how the signal of Pt FT gene moving by tobaccoes grafting. The results were as follows:1. By Pt FT::GUS fusion expression vector construction and Poncirus trifoliata(L.) Raf.and Nicotiana tabacum transformation, I got 11 transgenic tobaccoes and their phenotypic data. Transgenic Poncirus trifoliata(L.) Raf. regeneration buds are rooting. Comparing transgenic tobaccoes and wild tobaccoes, I found they were different in height, stem diameter, branch number, flowering time, the number of florets and seeds. Pt FT::GUS transgenic tobaccos were height lower,stem diameter smaller, more branches, earlier flowering, less florets and seed setting rate lower. I made GUS histochemical assays of Pt FT::GUS transgenic tobaccos, result in weak coloration.2. I planted Pt FT transgenic tobaccos( Pt FT DNA Pt FT1, Pt FT2, Pt FT3 and Pt FT4) and wild tobaccos(WT) to find different phenotypes among them. They were different in leaves number, numbers of stem nodes,height, branch number, stem diameter and flowering time. Probably because of extrons incomplete between Pt FT3 and Pt FT4, WT,Pt FT3 and Pt FT4 were the same in these phenotypes. Pt FT DNA, Pt FT1 and Pt FT2 were almost the same in these phenotypes indicating introns made no differences in leaves number, numbers of stem nodes,height, branch number, stem diameter and flowering time. The differences among WT, Pt FT3, Pt FT4 and Pt FT DNA, Pt FT1, Pt FT2 in leaves number, numbers of stem nodes, height, branch number, stem diameter and flowering time might result from gene insertion of Pt FT DNA, Pt FT1 and Pt FT2.3. I planted Pt FT transgenic tobaccos( Pt FT DNA and Pt FTc DNA) and wild tobaccos(WT) for gifting them to each other and themselves. I got their phenotypic data and identified them by m RNA. WT/WT flowered later than other six gifting types, indicating that there were signals moving between scion and stock changing the flowering time of scion.WT/FTDNA and WT/FTc DNA flowered more earlier than other five gifting types so I identified WT/FTDNA and WT/FTc DNA by m RNA. Research findings showed no m RNA of Pt FT gene detected either in 1cm or 5cm above the graft union. As a consequence, m RNA of Pt FT gene in stock moving was not likely to the reason that WT/FTDNA and WT/FTc DNA flowered more earlier than other five gifting types.