The Preliminary Research Of Developmental Effects In Arabidopsis From β-1,3-Glucanases Gene

β-1,3-glucanases is the large families involved in pathogen defense of plant. The degradation ofβ-1,3-glucan in cell walls is thought to contribute toward fungal cell wall destabilization as well as release of cell wall associated immune elicitors that further stimulate defense responses. They are involved in a wide range of developmental processes, but the mechanism is unknown. So we used the material of Arabidopsis thaliana, which was transfered the wheatβ-1,3-glucanases into it, to search the mechanism.The transgene plants GLU1 had the high auxin phenotype when they were young, dependability experiment demonstrated that was relatived to the auxin; Also treated with different concentration gradient of the plant hormone ABA, 6-BA and GA, the dependability analysis demonstrated that was also relative to these hormone.The plant anatomy experiment found that the vascular system has altered: in roots the cells of the xylem was reduce and the phloem was shrink, both the xylem and the phloem were reduced in the stem; the geotropism of the GLU1 plants was weak; Detect with the auxin report gene DR5-GUS, we found the in roots the concentration of auxin was reduced, but in cotyledon was increase. All these experiments showed that the PAT of the GLU1 plants had impaired.Compared the response to different auxin, we found that treated with NAA, the response of GLU1 plants and wild type was same, videliceted that there was no difference between the auxin outflux transporter; when treated with 2,4-D, the response of GLU1 plants was insensitivity, suggested that the auxin influx transporter had diversity; The IAA treated experiment also indicated there was difference between the auxin influx transporter; The results of RT-PCR indicated that both the expression of auxin outflux gene and influx gene has no marked change.Otherwise earlier flowering is the significant phenotype of the GLU1 plants, also there was some abortion. Treatment with the different illumination time and vernalization time, we found the earlier flowering phenotype had no relationship with Photoperiod pathway and Vernalization pathway, RT-PCR experiment demonstrated that the earlier flowering phenotype is relative with Gibberellins pathway and the Autonomous pathway: the expression of flower suppressing gene EMF1and EMF1 was reduced , which very important in the Autonomous pathway, the key gene of Gibberellins pathway SPY was also reduced. All these indicated that the earlier flowering of GLU1 plants relative to the Gibberellins pathway and Autonomous pathway.