The Effects Of Mobile Phone Radiation On Mouse Embryos Fibroblast

Accompany with the increase of users in mobile phone usage, the radiationcaused bymobile phone-related equipment has became the focus of attention. So far,there is no exact evidence indicate the existence of the mobile phone radiation couldinfect the cell bio-effects. This research was aim to study the affection of the mobilephone radiation on cell bio-effects of the mouse embryonic fibroblasts (NIH/3T3).The cells were exposing to the radiation on the real and simulative mobile phones,And the influence of the chemical carcinogen in the environment, which wouldprovide some new idea for the study on the relationship of the mobilephone radiationand the cell canceration in future.In short-term radiation, the NIH/3T3were exposed on900MHz/1800MHz realmobile phone and1800MHz simulation system which SAR(Specific AbsorptionRate)respectively2.0,3.0, and4.0W/kg.And the times of radiation were set1,2,4,6,8, and12hours respectively. In this study, the morphological changes of the cells andDNA damage could be detected.In the longtime radiation experiment, cells weretreated by promoting agent and carcinogens for24hours, and then exposed in the realmobile phone and sXc1800simulation system, which SAR respectively2.0,4.0W/kg. In the experiment, the time of mobile phone and simulation system were set to30min on and10min off. And the talking times should be keep4hours. All thisexperiment would be lasted for8weeks. After treatment by different mobile phone,the morphologic changes of the cells should be observed and the the cell number oftransformed foci, the damage of DNA, cell cycle and the expression of p53proteinwere deteted.The results showed that, in the short term irradiation, cell morphological changeswere not obvioused by Giemsa staining. The DNA of cell had minor damage by realphone for2h and the sXc1800simulation system which SAR≥2W/kg, but after12hours nomal culture, this damage disappeared. After irradiation by different radiationsource for8weeks, there had no significant changes on cytomorphology. And in theuse of promoting agent or adding irradiation, there is not significant difference also.But after treated by carcinogens, the transformed foci number has significantdifference. In the comet assay, the DNA of cell showed significant damage treated bycarcinogens and the damage degree of DNA mobile phone radiation was higher thansham groups. In cells, after treatment by carcinogens and join real mobile phone andSAR4.0W/kg irradiation, G0/G1decreased significantly, and the increase in G2/Mand S phase. The expression content of p53protein treated by carcinogens anradiation group were more higher than the other groups.In summary, short-term radiation on celluar DNA had a certain degree of damage, but this damage can be recovered quickly by the self-repair mechanism ofthe cell after12hours normal culture. When the NIH/3T3cell under normal state, orwas slightly damaged, and then exposed to the commonly used mobile phoneradiation, and would not have a significant impact. Only when the cells were sufferserious damaged, and coupled with long-term mobile phone radiation, may make thedamage worse. This study reveals the impact of mobile phone radiation on cells fromdifferent aspects, which provide the basis for the further study of mobile phone useharmful to human body and whether to initiate the normal cell cancerous.