Analysis Of Microbial Diversity In Deep-sea Sediments And Taxonomy Of A Bacterium From Marine Sediment

In this study, The environmental total DNA was directly extracted from three sediments in Pacific cobalt rich manganese crust (P20082), Pacific polymetallic nodule province (WS200801), and East Pacific Rise(TVG19). Three sediments bacterial and archaeal 16S rRNA gene libraries were generated. Phylogenetic analysis indicated that clones of three bacterial libraries can be divided into 15 phylotypes:Proteobacteria,Planctomycetes,Bacteroidetes,Acidobacteria,Verrucomicro bia,Deferribacteres,Gemmatimonadetes,Chlamydiae,Nitrospira,Actinobacteria,Chloro flex,Firmicutes, TM7,OP11,OD1.16S rDNA sequences from members of proteobacteria dominated three sediments samples, and it is revealed ahigh richness in these sediments bacterial communities. There archaeal librarieswere within phylums of Crenarchaeota andEuryarchaeota.A bacterium, designated C5, was isolated from deep-sea sediments of Pacific cobalt rich manganese crust (P20082).The strainC5 had 100% sequence similarity with Brachybacterium paraconglomeratum and is Mn oxidizing bacteria.A gram-negative designated strain CN85~T was isolated from marine sediment in East China Sea and was subjected to a polyphasic taxonomic characterization.On the basis of phenotypic and genotypic date, strain CN85~T represents a novel species of the genus Microbulbifer.