Chemiluminescence Detect System Of Miniaturized Total Analysis System

Miniaturized total analytical system has developed to be the hot-point in the researching fields of analytical chemistry during the past decade. Capillary electrophoresis, as a important branch of a Miniaturized total analytical system, which is a powerful technique for the separation due to its virtues of high resolution, efficiency, rapid separation, small analyte consumption and simple instrument. The chemiluminescence is one of the most sensitive detection methods in CE. CL optical systems are simple, requiring no light sources, and thus providing low background and high sensitivity. When coupled with CE, it can meet the demand of sensitivity and selectivity for the analysis of biological samples simultaneously. Most of the studies on this field focus on the design of instruments and development of new systems.The paper reviews predecessor's work, bases on the capillary electrophoresis chip principle, takes PMMA material for the production of CE; improves chemiluminescence detection chip structure on the basis of design "Cross" channel; developed electrophoresis chip samples; builds a chemiluminescent detection system using photodiode as a photoelectric detector, makes the circuit noise analysis and restrained circuit's noise through the shielding and grounding to achieve the input current sensitivity of 10pA and output voltage baseline of 2mV. The copper ion content of the aqueous solution is successful detected and the detection limit attained 10-9mol. Chemiluminescence intensity of is linear distributed with CuSO4 solution concentration from 0.5×10-7mol to 1×10-9mol. It verifies the rationality and feasibility of the chip design, which has set up a foundation for the next-step research.