Research Of Spectrum Detection Technology Based On The Miniaturized Total Analysis System PCR- Gene Chip

Miniaturized Total Analysis System,μTAS (or lab on a chip , LOC) is the process and step of biochemistry analyses, the biochemistry analyses laboratory of the " integrated function micro structure " on 100 millimeters of square retinue (or smaller) chip, having notable characteristics :detecting speed quickly, sample dosages small, exchanging amounts advanced. Common bio-chip is divided into two categories: micro-array chip and micro-fluidic chip.Detecting system is one of the key that the chip system studies. In a lot of detecting method, spectrum detecting as nicer selectivity, broader linearity range, the micro quantitative analysis and no destroyed detecting, becomes one of maximal detecting of sensitiveness technology in the detecting field. So with "functional integration and structural microfilm" the idea of spectral detection system has become to study and solve the hot topic in recent years in the world. Judging from the current domestic and foreign research and development related to the status quo, are at the initial stage of exploration and research.This paper for the above two categories of chip representative, and based on polymerase chain (PCR) reaction of the spectrum detection technology of gene chip, launched a related study, to further the "functional and structural integration of microfilm," study the establishment of a foundation.For micro-PCR gene-a-chip fluorescence spectrometry detection technology, launched on the following:We have set up a micro-flow control micro-channel dynamic detection system, its significance lies: in micro-biological fluorescence-a-chip using PCR, the analysis of the results of biological testing in the spectrum of micro-detection and integrated research, provide experimental basis. At the same time to prepare the chip, in order to optimize the best production process parameters, a study of laser-on-a-chip technology of biological fluorescence PCR analysis. In order to ensure the reunification of the fluorescence analysis in chip production technology and quality inspection, we study the standard template of the biological fluorescence PCR analysis. In order to make micro-controlled fluorescent PCR amplification cycle of actual flow velocity anastomosis with the theoretical design to ensure that the entire process of chip testing of the exact completion of the study fluorescence flow monitoring and control system. "Fluorescent real-time monitoring and control speed system"is the first described in the literature of several micro-fluid flow measurement methods at home and abroad.For micro-array gene chip PCR method visible spectrum of detection technologies, launched on the following:According to nano-probe micro-array PCR gene chip testing spectrum characteristics, carried the research on nano-bio-chip probe the "double optical amplification method" of the sediments and enhanced resolving power of spectral detection method, get resolving power and System solutions. LED semiconductor materials and the use of optical-guided excimer laser system and including micro-processing, laser processing technology, design research and development of the high uniformity of thin flat light scattering system.