| [Objective]Water maze experiment provide theoretical and practical measure for the explore and research of mankind memory and medicine for brain, through studying the effect of the surroundings to the animal's ability of study,memory,recognition and identification. It's widely used in psychics' research. At present, the economical system used in water maze experiment has the conflict with the precise system: The economical facility is not precise and the precise system is not cheap enough. The economical facility is imprecise for its bad material and outdated method, while the precise one is costly for its special hardware and software. In addition, whichever device has no ability to expand function to adapt to the need of water maze experiment's progress.Based on the research of this experiment's theory and technique, a system using a programmable language LabVIEW is designed with digital camera,PC and IMAQ card. This system makes the water maze experiment's image collection and deposition more precise and reliable with new functions in low cost. Agility, the common character in VI, is the reason that the system can be used in other fields by adding new module and expanding functions.[Methods]LabVIEW programmable language is the primary program language in the system. At the beginning set the parameters of image collection, including the luminance and field of vision. Then test the real distance between one pixel and the next in the CCD using a standard module. Run the main program, save the running image as the named AVI file, dispose every frame in real time, distinguish and save the middle point of the object. With the disposition of the coordinate, calculate the TD,TT,TS,TP and display the waveform of TS. After the experiment is complete we can accomplish the function of displaying the total time in every quadrant,replaying the moving track and all indication with the saved coordinate data.This program can be used in the test of blood stream speed of micro-circulation by changing some module and parameters. First distinguish the blood cell by the luminance and bulk of blood cell from surroundings. Then calculate the relative moving distance of the cell which is nearest to the middle point of image in a short time and judge if the number of it has changed. If the number has changed, then reselect another blood cell, reapply upper program.For the limitation of experiment devices, only static blood stream analysis has been done.[Result]An image collection system programmed by LabVIEW can collect,analyze and save data from water maze experiment precisely. The result is saved as two types: AVI file and LVM file. AVI file record the whole image which can be replayed by display software and analyzed by other software. LVM file save the coordinate data of the distinguished point which is saved as a pair of number and will be read to replay the track.This system has ability to distinguish and locate blood cell in static blood stream picture and will be evaluated in the dynamic pictures.[Conclusions]The system based on LabVIEW for Morris experiment's image collection can save and analyze data obtained from experiment with more functions and lower cost than other image collection system. The blood stream test system based upper system has feasibility and good future. |
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