Research Of A Multi-parameters Bio-sensor For Blood Analysis

With the chances of the population age structure and the requirement of improvement in its health level, some enhancements to the clinic medical are expected. Specialist point out the cure medical will be replaced by preventive medical in the future. Therefore, effective, simple and convenient determination of disease omen in advance, such as the change of blood glucose, Total Cholesterol and other biochemical characters, has attracted a great deal of interest in the research field of medical.Biochip technique is applied to blood analysis and measurement in this thesis. A multi-parameters biosensor used for blood analysis in medicine has been researched and fabricated, by which the concentration tests of Blood sugar, Total Cholesterol, Acetone body and Lactic acid in only one drop of blood are made simultaneous.The main research work is introduced briefly, as follows:Since micro-dosage of liquid is tested on biochips, electrodes need to be miniaturized and the reaction current is very small. Therefore IDA microelectrodes are applied to this biosensor, because their particular structure is very effective in enhancing the response current and measurement sensitivity. The IDA microelectrodes are fabricated by photography, film deposition and other microfabrication techniques, and two process flows based on lift-off and etching are carried out.The current of chemical reaction between enzymes and blood contents depends on conditions. For different blood parameter, enzyme solutions with ratable substance are formed and enzyme mixtures are modified on the surface of different microelectrodes.Blood distribution structure is designed and fabricated, to distribute blood and isolate reaction areas.A multi-channel instrument for current measurement is designed and developed to test reaction current.The system characteristics of the biosensor are tested and its qualification test is carried out.