Study On Gold - Iron Lock To Root Bark Based On Toxicology And Analgesic Anti - Inflammatory Efficacy

Objective:Psammosilenes Radix, from Psammosilene tunicoides W. C. Wu et C. Y. Wu, is one of the traditional miao herbs commonly used in traditional folk medicine, for the treatment of rheumatism, inflammation, traumatic injury, bleeding wound, swelling and pain. It is an integral part of Yunnan Baiyao. Through reviewing literatures and summarizing the early work of our team, we have found:the problem of whether or not to peel the root bark exist in Psammosilene tunicoides. How about similar medicinal materials in the Chinese Pharmacopoeia. Then, it recorded toxic long time ago. However, few information is focused on its toxicity. How the toxicity exactly works? Whether there is a connection between the root bark and toxicity or drug action? In order to solve the above problems, this paper have following studied:compare the differences in the acute and sub-acute oral toxicity of Psammosilenes Radix (PR), Psammosilenes Radix with Cortex (PRC) and Psammosilenes Cortex (PC); the differences in analgesic and anti-inflammatory effects among the PR, PRC, PC; discuss whether the root bark requires peeled when used, based on the toxicity test and analgesic and anti-inflammatory experiments.Methods:Through reading a large number of ancient and modern literatures about the peeled problem of the different medicinal materials, and summarize. Using the acute oral toxicity test to compare the acute toxicity of PR, PRC, PC. Using the sub-acute oral toxicity test to compare the sub-acute toxicity of PR, PRC, PC. Using the hot plate test in mice to compare the analgesic effect, and the AA model in rat to compare the anti-inflammatory effect.Results:1 Problems of peeled traditional Chinese medicines in Chinese Pharmacopoeia.The 2010 edition of Chinese Pharmacopoeia contains 67 kinds of medicinal materials. Most of them recorded in ancient literatures. But its specific reasons are not recorded in detail. The reasons for peeling generally include:to facilitate the extraction of the effective components from medicine, remove the non medicinal parts, beautify the appearance or facilitate the slicing. Modern studies have focused on the research of chemical composition, pharmacological activity and toxicity to determine whether they should be peeled, the results may be different, it still needs further proof.From the above, modern research about peeling problem still lacks of systematic. First, a lot of disputes can not be solved properly only from the chemical composition and pharmacodynamic evaluation. Then, the key evaluation index of the peeling problem is not yet clear. In addition, we didn’t consider to classify the problem according to the clinical application. Therefore, it is necessary to establish a set of scientific and systematic research system for evaluation of the peel of Chinese medicinal materials. This system can provide a valuable reference to solve the problem of the peeling problem in harvesting and processing, even can be used for the shell and heart issues.2 Acute oral toxicity of the aqueous solution from PR, PRC, PC.Single dose administration of mice cause the death of three groups. The physiological and pathological changes are obvious. The acute toxicity LD50 values of the three groups were respectively 4.6382 g·kg-1,4.8471 g·kg-1,6.4032 g·kg-1. The acute toxicity LD50 value of PR is 92 times of clinical dosage. PR and PRC groups can first inhibit weight gain, then gradually restore. The PC group can also inhibit weight increase, but can not be restored. The main toxic target organs include lung, spleen, stomach. The PR and PRC groups can cause kidney damage, when PC group cause liver injury and intestinal irritation.3 Acute oral toxicity of the aqueous solution from PR, PRC, PC.Body weight, eating, pathological changes obviously of PR, PRC, PC groups after 28 days administration. Female rats weight reduced obviously, when male rats weight has no obvious change. Then, Food intake have varying degrees of reduction in both sexs. Comprehensive changes of blood and serum index, viscera coefficient, histopathologic, three samples all cause interstitial pneumonia, glomerular filtration function damage, high dose group was the most serious. In addition, the high dose group of PR cause liver cell damage and mild irritation of the stomach, the high dose group of PRC cause congestion to atrophy of the heart and mild irritation of the stomach, the high dose group of PC cause congestion to atrophy of the heart. It has slight difference of subacute toxicity. Then, 0.3g·kg-1 can be used as a safe dose in rats, which is 9 times of the clinical dosage.4 Analgesic effect of the aqueous solution from PR, PRC, PC.In the hot plate test model, three samples with seven days administration can all raise the pain threshold of mice, show that three samples produce analgesic effect on this model.5 Anti-inflammatory effect of the aqueous solution from PR, PRC, PC.In the adjuvant arthritis models, three samples could all significantly inhibit the secondary side toe swelling degree, and significantly decreased the content of IL-1β and TNF-a of the AA rats. High dose groups show stable effect. Thus, three samples produce significantly anti-inflammatory effectst on this model.Conclusion:Through our research, we clarified the acute and subacute toxicity of the aqueous solution from PR, PRC, PC. It has slight difference of toxicity, and they all have analgesic and anti-inflammatory effects. Combined with the actual difficulty of peeling and the market situation, we believe that the PRC and PC can both be used as the medicine. We can not peel the root bark when processing. Psammosilene tunicoides, moreover, has certain toxicity, clinical use should not be excessive.