| Objective:Lumbar Disc Herniation is a common and frequently encountered disease in the clinic.Its main symptoms are low back pain and sciatica.lt can influence the normal working and studying of people’s life.It is popular in the clinical due to its obviously curative effect on the LDH.This study focuses on the effect of acupotomy nerve stimulation to the changes of blood flow of the dorsal root ganglion in L5 and TXB2 and 6-K-PGF1α in plasma of rats with lumbar disc herniation,so as to explore the effect and the possible mechanism of acupotomy on the microcirculation of the dorsal root ganglion in L5 and provides experimental basis in clinical treatment of the acupotomy.Methods:A total of 40 male Sprague-Dawley(SD) rats were randomly divided into four groups(n=10): blank group,model group,acupotomy group,and electroacupuncture group.All groups of rats were test the hind limbs of thermal stimulation threshold before the modeling, after the modeling, and after the treatment respectively. When the autologous nucleus pulposus of coccygeal vertebral was injected to the epidural cavum of rat by lumbar puncture,the non-compressive model of experimental lumbar nucleus pulposus prolapse was built in model group,acupotomy group,and electroacupuncture group.For the acupotomy group,3 day after modeling,both the acupotomy and the electroacupuncture group was treated every other day three times in a week.After the treatment, laser Doppler flowmeter was used to record the dynamic changes of blood flow of the dorsal root ganglion in L5,and got the plasma of rats of all groups to test the changes of TXB2 and 6-K-PGF1α by ELISA.Results:(1)Before the modeling,the hindpaw thermal withdrawal latencies of all the groups were compared. No significant difference in withdrawal latency was observed between the groups(p>0.05).After the modeling, the datum of all the groups were compared.Compared to the blank group,the mean threshold value of other three groups were reduced and showed a gradually decreasing pattern (P<0.01).In contrast with model group,the data of the acupotomy group and electroacupuncture group have no significant difference.That shows the models are successful.After the intervention of acupotomy and electroacupuncture, the datum of all the groups were compared.Compared to the model group,the datum of acupotomy group were increased significantly(p<0.01),the electroacupuncture group had no difference.(2)Compared with the blank group,the blood flow of model group and electroacupuncture group decreased significantly difference(p<0.01), compared with the model group,the acupotomy group increased significantly (P<0.01); compared with the electroacupuncture group,the acupotomy group increased significantly(P<0.05).(3) The TXB2 level in the model group, acupotomy and electroacupuncture group were significantly higher than that in the blank group (P<0.05 or P<0.01). Compared with the model group, there was a decrease of TXB2 in both acupotomy and electroacupuncture group(P<0.05 or P<0.01). The 6-K-PGF1α level in the model group were significantly lower than that in the blank group(P<0.01).Compared with the model group,there was a significant increase in both acupotomy and electroacupuncture group(P<0.01 or P<0.05). The level of the ratio of TXB2 and 6-K-PGF1α were same to that of TXB2.Conclusions:(1) The intervention of acupotomy nerve stimulation can increase the blood flow of DRG in rats with lumbar disc herniation significantly,and can also regulate the balance of between TXB2 and 6-K-PGF1α,which is closely related to the vasomotor state of the blood vessels.(2) The mechanism of improving the microcirculation of the DRG may be through decreasing the TXB2 and regulating the balance of TXB2 and 6-K-PGF1α. |
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