| ObjectiveThe experimental study in cold stagnation syndrome of dysmenorrheal rats as the research object, according to the pathogenesis of primary dysmenorrhea, related to cell palace acupuncture point(SP6,RN4) as the starting point,compare different acupoints on coldstagnation syndrome of dysmenorrheal rats writhing respons, uterine microcirculation,uterine tissue prostaglandin F2α (PGF2α) , prostaglandin E2 (PGE2) , content and the ratio of PGF2α/PGE2, the prostaglandin F2α (PGF2α), prostaglandin E2 (PGE2) receptor mRNA expression level,the MLCK , preliminary discussion on the mechanism of action of acupuncture pain alleviate cellular palace, at the same time to explore the analgesic effect of different acupuncture points differences.MethodsThis experiment selects 72 only estrogen interphase SD female rats,divided into following 4 groups according to random method, including:saline group, model group, Sanyinjiao(SP6) group, and Guanyuan(RN4)group, each group of 18. Except for saline group, the rats in other groups were given to injection of estradiol benzoate oxytocin, after the injection, freeze pwere put into the ultra-lowtemperature freezers for 4hours, we opened the freeze rventilationat 2hours for 5 seconds and the freezer ismaintained at a temperature of -25℃. Saline group were given the same dose of saline daily. To observe the writhing latency and writhing number and writhing scorein rats; The observation group 5,10 and 20min uterus microcirculation of rats; Using ELISA method to detection of uterus tissue in rat prostaglandinF2α (PGF2α),prostaglandin E2(PGE2) content; Using rt-pcr method to detect the rat uterus prostaglandinF2α (PGF2α), prostaglandin E2(PGE2) receptor mRNA expression level in the organization; Using ELISA method to detection of rat uterine smooth muscle myosin light chain kinase(MLCK) content.Results1 Effect on writhing response of on different acupoints of cold stagnation syndrome of dysmenorrheal ratsCompared with saline group, the writhing latency in model group was significantly shortened (P<0. 01) , the writhing number and writhing score in model group was increased significantly (P<0. 01).Compared with the model group, the writhing latency in Sanyinjiao (SP6) group was significantly longer, the writhing number and writhing score decreased significantly(P<0.01); the writhing latency in Guanyuan (RN4) group wassignif icantly longer, the writhing number and writhing score in decreased significantly (P<0.05).Comparison between the acupuncture group, writhing latency,writhing number and writhing score, there was no significant difference(P>0. 05).2 Effect on uterine microcirculation of on different acupoints of cold stagnation syndrome of dysmenorrheal ratsCompared with saline group, micro-vessel diameter, cap diameter capillary contraction (P<0.01),obviously the cap number decreased significantly (P<0. 01), micro-vessel clarity decreased significantly(P<0.01), micro-vessel colordeepened obiously (P<0.01), significantly slow or stop blood flow(P<0.01).Compared with model group, Sanyinjio(SP6) at various time points micro-vessel diameter expansion,statisitically significant(P<0.05,P<0.01), cap diameter capillaryexpansion statisitically significant(P<0. 05,P<0. 01) , micro-vessel clarity resolution obviously improved(P<0. 05,P<0. 01) ,micro-vessel color obviously improved (P<0. 05, P<0. 01),significantly speed up the blood flow (P<0. 01);Guanyuan(RN4) ofuterine micro-vessel diameterlOmin after the start of the acupuncture point expansion (P<0. 05),micro-vessel clarity resolution obviously improved "(P<0.05,P<0.01,P<0. 05), 10min time micro-vessel color obviously improved (P<0.05),5 min the the blood flow obviously accelerated(P<0.01).Comparison between the acupuncture group,Sanyinjiao (SP6) of 10,20min, micro-vessel diameter expansion in the Guanyuan(RN4)(P<0.01),Sanyinjiao (SP6) 5,10min the cap diameter expansion in the Guanyuan(RN4) (P<0.05),Sanyinjiao(SP6)10, 20min micro-vessel color obviously improved(P<0.05, P<0.01), Sanyinjiao (SP6) 10,20min significantly slow or stop blood flow in the Guanyuan (RN4) (P<0.01).3 Effect on uterine prostaglandin on different acupoints of cold stagnation syndrome of dysmenorrheal rats3. 1 Effect on uterine PGF2α、PGE2 levels and the ratio of PGF2α/PGE2 on different acupoints of cold stagnation syndrome of dysmenorrheal ratsCompared with saline group, PGF2αof in model group was increased significantly(P<0. 01) and the ratio of PGF2α/PGE2 decreased significantly(P<0. 01).Compared with model group, PGF2α in Guanyuan (RN4) decreased significantly(P<0. 01), the ratio of PGF2α/PGE2 decreasd significantly(P<0. 01).Comparison between the acupuncture group,Guanyuan(RN4) PGF2α and the ratio of PGF2α/PGE2 decreasd significantly(P<0. 05, P<0.01).3. 2 Effect on uterine PGF2α、PGE2 recetpor mRNA expression level and the ratio of PGF2α/PGE2 on different acupoints of cold stagnation syndrome of dysmenorrheal ratsCompared with saline group, PGF2α,PGE2 receptor mRNA epression level of in model group was increased significantly(P<0. 01).Compared with model group, PGF2αreceptor mRNA epression level in Sanyinjiao(SP6) decreased significantly (P<0.05);PGE2 receptor mRNA epression level in Sanyinjiao (SP6) and Guanyuan (RN4) decreased significantly(P<0. 05,P<0.01).Comparison between theacupuncture group,uterus PGF2α,PGE2 receptor mRNA epression level and the ratio of PGF2α/PGE2 did no significant difference (P>0. 05).4 Effect on uterine MLCK on different acupoints of cold stagnation syndrome of dysmenorrheal ratsCompared with saline group, MLCK of in model group was increased significantly(P<0. 01).Comepared with model group, the acupuncture group ofuterine MLCK content has no abvious change there was nosignificant difference(P>0. 05).Comparison between the acupuncture group, uterine MLCK content has no abvious change, there was nosignificant difference (P>0.05).Conclusions1 Systemic freezing can cause cold stagnation syndrome in rats; to combined preparation of dysmenorrhea model with cold stagnation model we successfully established the dysmenorrhea rat model with cold stagnation syndrome.2 All stduy acupoints (SP6, RN4) analgesic effect in dysmenorrheal model rats.3 All stduy acupoints (SP6, RN4) can obviously improve dysmenorrheal model rats of uerine microcirculation status.4 Speculated that acupuncture may be by adjusting the pressure duing contraction and relaxation of isolated uterine smooth muscle of substances, therebyalleviating uterine smooth muscle spasticity.5 Different acupoints have different analgesic action. Sanyinjiao (SP6)isrelatively Guanyuan (RN4) analgesic effect obviously.From what has been discussed above, primary dysmenorrhea model neural endocrine disorders and the level of uerine microcirculation dysfunction, is the part of the mechanism of the disease. Part of the mechanism of acupuncture on the treatment effect of primary dysmenorrheal is by regulating the body of prostaglandins level disorder,thus improving microcirculation dysfunction and pain relief. Compared with two acupoints and the adjusting effect of different cell palace , SP6 each indes were significantly,explain SP6 has the effect of regulating cell palce specificity, the specificity is relative. |
