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Research On Micro-Sized, Highly Intensive And Multi-LED Integration Technique And Process Equipment For Biochip Fluorescence Detection

Posted on:2013-10-18Degree:MasterType:Thesis
Country:ChinaCandidate:Q LiFull Text:PDF
GTID:2248330362968492Subject:Physics
Abstract/Summary:PDF Full Text Request
Biochip realizes all the biological engineering analysis process and the completelaboratory functions on the miniaturization basis. It integrates all the functionsincluding sampling, dilution, adding reagents, reaction, separation and detection inone chip, known as the ‘Lab on a chip’. Its scientific significance and superiorityare reflected in its functional integration and the structure of microfilm. Thefluorescence spectroscopy detection is a detection technique in which excitation lightsource is used to excite objects to create fluorescence, based on which qualitative andquantitative analysis could be conducted. The technique has become one of the mostwidely-used and most sensitive detection techniques in the biochip domain due to itsadvantage of being well targeted, non-destructive and allowing for qualitative andquantitative analysis on a micro scale. In the current practice of biochip fluorescencedetection, a personal computer-sized fluorescence detection device is used to detect ona coin-sized chip. The nature of such a practice is contradictory to the advancedessence of biochips. Therefore, it is urgent and scientifically important to solve theabove bottleneck problem. In recent years, the minimization of fluorescence detectionsystems has become a world focus, but its research remains at a very primitive stageboth domestically and abroad.The over-sized excitation light source and auxiliary structure in the fluorescencespectroscopy detection are one of the main barricades that prevent the integration andminimization of the system. Therefore, the integration and minimization of the systemrequires the development of an excitation light source for micro-fluorescence spectradetection system, which is small enough to be embedded onto chips and could satisfythe intensity requirements for bio-techniques. This is in accordance with biochip’sidea of functional integration and structure of microfilm.Based on the above requirements, the following research has been conducted with anaim to develop an excitation light source for micro-fluorescence spectra detectionsystem, which is small enough to be embedded onto chips a nd could satisfy theintensity requirements for bio-techniques.(1) An integration technique with micro-sized, highly intensive and multi-LEDfeatures is raised and detailed solution proposed. The light source is smallenough to be embedded onto chips and could satisfy the intensityrequirements for bio-techniques, and its spectral characteristics could bemanaged and adjusted. This technology has been authorized by the nationalinvention patent.(2) In order to realize the integration technique with micro-sized, highly intensive and multi-LED features, a multi-LED steric encapsulation device isdeveloped, the first of the kind to cater for the market’s need. This devicecan produce a3-D light source structure with precise control over relevantparameters. The device comprises of three parts, the mechanical system, theelectrical system and the software programs. The mechanical system iscomposed of a backplane and four stepper motors; the electrical systemincludes the MCU AT89C52, LCD12864and ULN2003, stepper motor28BYJ-48and4buttons; the software program is set upon the MCU torealize the overall control. This device lays the foundation for furtheranalysis of the3-D condenser features of the micro-sized and high intensitylight source. This technology has been passed by the national inventionpatent preliminary.Based on the technical solution of micro-sized, highly intensive and multi-LEDintegration and by using the multi-LED steric encapsulation device, several realmicro-sized and highly intensive light source is produced, which proved thepracticality of the technical solution and laid a foundation for the further research onmicro-optical systems featuring the following: a smaller volume, an intensity meetingthe requirement for biological detection of biochips, allowance for spectral selection,and micro-lens condenser system on luminous surface.
Keywords/Search Tags:for biochip, micro-fluorescence spectra detection, micro-sized andhighly intensive, multi-LED integration, multi-LED steric encapsulation device
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