Effects Of Acute Bouts Of Swimming Exercise On AMPKα2 Expression In Mouse Muscle

The 5' -AMP-activated protein kinase(AMPK) is a multisubstrate serine/ threonine protein kinase which is ubiquitously expressed and functions as an intracellular fuel sensor activated by depletion of high energy phosphate compounds.The overall function of AMPK is in response to cellular energy deprivation to increase the potential for ATP production while concurrently decreasing cellular energy consuming anabolic processes.Theα2 isoform is by far the more abundant in skeletal muscle, and is very important for the AMPK activity.The purpose of this study was to determine the effects of the exercise (swimming) time on the AMPKα2 gene and protein expression in mice skeletal muscle,and the relationship between the expression of AMPK and the blood glucose,muscle glycogen and blood insulin.AMPKα2 expression was measured after the same intensity swimming exercise at different times in mice quadriceps muscles of the C57 mice. 90-minute swimming and no more than 3-hour swimming activated theα2 isoform in quadriceps muscles.The control group had none exercise.Blood glucose,quadriceps muscle glycogen and blood insulin also were measured. The 90-minute and the exhausted swimming exercise resulted in a greater increase of muscle AMPKα2 expression compared with the control group, and the AMPKα2 expression was higher after exhaustive swimming than the 90-minute swim.The results suggested that the different exercise times had effects on the skeletal muscle AMPKα2 expression.Our study also found that there was a negative correlation between the AMPKα2 expression and the blood glucose level.