Effects Of Acanthopanax Senticosus Use On Carbohydrate Matoblism And Serum Levels Of Related Hormones After Exercise In Rats

Objective: To preliminarily explore the mechanism of glycogen synthesis improved by Acanthopanax Senticosus through investigating its effects on the glycogen content in skeletal muscle and liver and serum levels of related hormones after a glycogen-depletion exercise in rats. Methods: 136 male SD rats were divided into 5 groups,including a rest control group (RCG) of 8 rats,a training control group (TCG) of 32 rats,three groups which were respectively supplied with sucrose 6g/Kg/d (SG),Acanthopanax Senticosus 500mg/Kg/d (AG) and both supplies (SAG) within 30 minutes after a high-intensity intermittent swim training and an endurance swim training every day. After 7 days of training and supplement,rats except RCG were made to perform a glycogen-depletion exercise on the 8th day. Muscle Glycogen, Liver Glycogen, Serum Glucose, Serum Insulin and Serum Glucagon were tested at four different time points before and after the glycogen-depletion exercise (before exercise,1 hour,4 hour and 12 hour after exercise). Results:⑴Serum glucose of SAG was significantly higher than that of TCG or SG at 12 hour after exercise(P<0.05). There were no significant difference between other this time and between all at the other three time points.⑵For the content of muscle glycogen, AG was significantly higher than TCG before exercise and at 12 hour after exercise(P<0.05). SAG increased significantly as compared with SG before exercise and at 4 hour after exercise(P<0.05), but at 12 hour after exercise there was a rising trend between these two(P=0.066).⑶For the content of liver glycogen, interestingly AG was lower significantly than TCG before exercise(P<0.05), but higher during recovery after exercise (P<0.05). SAG increased significantly as compared with SG before and after exercise(P<0.05).⑷For serum insulin,there was no significant difference between AG and TCG before and after exercise(P>0.05).But before exercise and at 4hr,12hr after exercise SAG was higher significantly than SG(P<0.05).⑸For serum glucagon, two groups supplied with Acanthopanax Senticosus decreased significantly as compared with two without supply at 1hr,12hr after exercise(P<0.05).At the other two time these groups showed no significant difference(P>0.05).Conclusions:⑴The supplement of Acanthopanax Senticosus alone can significantly increases the content of muscle glycogen at rest and promotes glycogen synthesis in both skeletal muscle and liver during recovery after exercise. Promoted secretion of glucagon from 1 hour to 4 hour after exercise and its inhibited secretion from 4 hour to 12 hour after exercise by Acanthopanax Senticosus seem to have contributed to improving glycogen replenishment in recovery. No effect of Acanthopanax Senticosus on serum insulin and glucagon at rest has nothing to do with increased content of skeletal muscle glycogen before exercise by Acanthopanax Senticosus.⑵The supplement of Acanthopanax Senticosus together with sucrose can provide such a further advantaged circumstance for glycogen synthesis(increased serum level of insulin at rest and its improved secretion after exercise; the same bidirectional effect on glucagon after exercise as it alone;slower decline of serum glucose to offer enough material)that glycogen synthesis in both skeletal muscle and liver before and after exercise is dramatically enhanced.The supplement of both Acanthopanax Senticosus and sucrose may play a significant role on strengthening sport capability.