The Experimental Study Of Movement, Trifolium Pretense L And Formononetin Xanthate Affect Hyperlipidemia Rats' Kidney Tissue

Purpose: Discussing the protective function and possible mechanism ofthe aerobics, Trifolium Pretense L and Formononetin xanthate when they are harminglipide kidney.Methods: Selecting 70 three-month-age rats (which are provided by Xi'an JiaotongUniversity Medical school animal administrative center), the body weight 220±20g.dividing the test rats into 7 groups randomly, each group has 10 rats: the first group ispeace-comparison-group; the second group is pinguid-comparison-group; the thirdgroup is pinguid-and-aerobics-group; the fourth group is pinguid-and-Formononetinxanthate-group;the fifth group is pinguid-and-Formononetin xanthate-and-aerobics-group; the sixth group is pinguid-and-Trifolium Pretense L-group; and the seventhgroup is pinguid-and-Trifolium Pretense L-and-aerobics-group. Giving the peace-comparison-group basic feedingstuff, drinking freely; feeding the other six groups withpinguid feedingstuff which contain 2% cholesterin, 10% lard and 0.5% cholic acidsodium. Four weeks after that, building up pinguid model. From the fifth week, thesports burthen of aerobics-group and medicament-and-aerobics-group is 5 days perweek. The step-up speed is 3m/min, its time is 5 minutes. The step-up course willcontinue 60 minutes till the speed reach 20m/min,keep this movement intensity.Thetraining course will last four weeks. Basing on pinguid feedingstuff, filling 13.5mgfeedingstuff per day into the stomach for the Formononetin xanthate-group andFormononetin xanthate-and-aerobics-group rats, and 400mg feedingstuff per day forTrifolium Pretense L-group and Trifolium Pretense L-and-aerobics-group rats. For thepeace-comparison-group, instead of brine with 2ml per day once. Observe the influenceof aerobics, medicament and medicament-and-aerobics to the rats' ecumenic conditionand blood fat; the expression to the rats' renal glomerulus membrane cell multiplication,extra cellular matrix proliferation, renal canaliculus simple fabric cell infiltration andrenal tissue collagen; the expression to renal organization growth genes (TGF-β,VEGF, CTGF), adherency molecule (ICAM-1, VCAM-1), chemotactic protein(MCP-1,OPN), nucleus duplication gene-κB, framework protein (Desmin,Vimentin,α -SMA) and cell perish protein (Bax,Bcl-2). All data are managed by the softwareSPSS13.0 for Windows. The statistics methods contain One-Way ANOVA method,two-comparisons method of Independent-Samples T Test. The results are expressed withaverage±Standard deviation((?)±SD).The significance difference chooses the level of(P＜0.05) and (P＜0.01).Results: Compare the pinguid-comparison-group to the peace-comparison-group,the pinguid-comparison-group rats'hair is coarseness, lackluster and desquamating, andgoing with these phenomenas such as movement decreasing and dispirited.Thepinguid-comparison,group rats' avoirdupois are obvious heavier than the peace-comparison-group (P＜0.01); These renal fat pad and epididymis fat pad are obvioushigher than the peace-comparison-group (P＜0.01);The compare between the other twogroups about kidney fat pad/avoirdupois and epididymis fat pad/avoirdupois hasremarkable difference too.The significance of pinguid-comparison-group rats' bloodserum TC and LDL is higher than the peace-comparison-group (P＜0.01),The pinguid-group rats' renal glomerulus are large obvious, the specific weight increase ofmembrane cells in the ball, part atrophy ingredient leaf-shape change, renal glomeruluspouch membrane accumulation, the membrane area increases the width; tubulus renalisepithelial cell vacuole type denaturation, between nature swelling, has the massivesingle nuclear cell infiltration. The kidney organization has obvious red dyes (VG) andthe blue dyes (Masson) collagen proliferation phenomenon.The pinguid-group rats'kidney organization TGF-β, VEGF, CTGF, ICAM-1, VCAM-1, MCP-1, OPN, NF-κB,Desmin, Vimentin,α-SMA, Bax expression increase (P＜0.01), Bax/Bcl-2 ratio toincrease (P＜0.01).Aerobics-group compares to the pinguid-comparison-group, the kidney tissuechange obviously to reduce. In the kidney tissue has a part of renal glomerulus to belarge, has the few vacuolization, in the ball is partially the membrane cell light moderateincreases; The tubulus renalis epithelial cell has the part of vacuole type denaturation,has the few single nuclear cell infiltration. The Masson dyeing and the VG dyeingdemonstration interrenal nature and in the renal glomerulus matrix has few red dyes andblue dyes with the collagen textile fiber. Aerobics to the hyperlipemia rats' kidney tissueTGF-β, CTGF, VCAM-1, MCP-1, OPN, NF-κB, Desmin, Vimentin, a-SMA, Baxexpression has the obvious inhibitory action, quite is obvious with the pinguid-comparison-group reduces (P＜0.01). But to VEGF, ICAM-1 in kidney tissue's expression is not remarkable inhibitory action (P＞0.05).Feeding-Formononetin xanthate-group compare with the pinguid-comparison-group, the kidney tissue change obviously to reduce. In the kidney tissue has a part ofrenal glomerulus to be large, has the few vacuolization, in the ball is partially themembrane cell light moderate increases. The tubulus renalis epithelial cell has the partof vacuole type denaturation, has the few single nuclear cell infiltration. The Massondyeing and the VG dyeing demonstration has few red dyes and blue dyes collagen fiberin the interrenal nature and in the renal glomerulus matrix.The Formononetin xanthatetreatment to the hyperlipemia rats' kidney tissue TGF-β,VEGF, VCAM-1, MCP-1, OPN, ICAM-1, Desmin,α-SMA, Bax expression has the obvious inhibitory action, quite is obvious reduces (P＜0.01).But to CTGF, NF-κB, Vimentin in kidney tissue'shigh expression has not remarkable inhibitory action (P＞0.05).Feeding-Formononetin xanthate+aerobics-group to compare with the pinguid-comparison-group, the kidney tissue change obviously to reduce.The kidneyorganization internal friction sees the renal glomerulus to be large, in the ball is themembrane cell increases mildly; Tubulus renalis nature has the few single nuclear cellinfiltration. The Masson dyeing and the VG dyeing demonstration has few red dyes andblue dyes collagen fiber in the interrenal nature and in the renal glomerulus matrix.Feeding-Formononetin xanthate+aerobics-group to the hyperlipemia rats' kidney tissueTGF-β, VEGF, ICAM-1, CTGF, VCAM-1, MCP-1, OPN, Desmin, Vimentin,α-SMA, Bax expression has the obvious inhibitory action, quite is obvious with thepinguid-group reduces (P＜0.01), is especially most obvious to the ICAM-1 suppressioneffect, but to kidney tissue NF-κB high expression have no remarkable inhibitory action(P＞0.05).Feeding-Trifolium Pretense L-group to compare with the pinguid-comparison-group, the kidney tissue change obviously to reduce. In the kidney tissue fias a part ofrenal glomerulus to be large, has the few vacuolization, in the ball is partially themembrane cell light moderate increases; The tubulus renalis epithelial cell has the partof vacuole type denaturation, has the few single nuclear cell infiltration. The Massondyeing and the VG dyeing demonstration has few red dyes and blue dyes collagen fiberin the interrenal nature and in the renal glomerulus matrix. The Trifolium Pretense Ltreatment to the hyperlipemia rats' kidney tissue TGF-β, VEGF, VCAM-1, MCP-1, OPN, CTGF, NF-κB, ICAM-1, Desmin,α-SMA, Bax expression has the obvious inhibitory action, quite is obvious with the pinguid-group reduces (P＜0.01), isespecially most obvious to the ICAM-1 suppression effect, but to Vimentin in kidneyorganization's high expression has no remarkable inhibitory action (P＞0.05).Feeding-Trifolium Pretense L+aerobics-group to compare with the pinguid-comparison-group, the kidney tissue change compares pinguid-comparison-groupobviously to reduce. The kidney tissue internal friction sees the renal glomerulus to belarge, in the ball is the membrane cell increases mildly; Tubulus renalis nature has thefew single nuclear cell infiltration. The Masson dyeing and the VG dyeingdemonstration has few red dyes and blue dyes collagen fiber in the interrenal nature andin the renal glomerulus matrix. Feeding- Trifolium. Pretense L+aerobics-group to thehyperlipemia rats' kidney tissue TGF-β, VEGF, ICAM-1, CTGF, VCAM-1, MCP-1, OPN, Desmin, Vimentin, NF-κB,α-SMA, Bax expression has the obvious inhibitoryaction, quite is obvious with the pinguid-group reduces (P＜0.01), to ICAM-1, the OPNsuppression effect is especially most obvious.Feeding-Trifolium Pretense L-group to compare with the Feeding-Formononetinxanthate-group, creates the kidney tissue change by the lipin deposition all have certainprotective function. But in creates the kidney tissue cell factor release aspect to the lipindeposition to have the difference: Feeding-Trifolium Pretense L-group effectsignificance surpasses the Feeding-Formononetin xanthate-group in suppressing theexpression of kidney tissue cell factor VEGF, TGF-β, CTGF, NF-κB and a-SMA(P＜0.01), but two groups in suppressing the expression of kidney tissue cell factorICAM, VCAM, MCP-1, OPN, Desmin and vimentin don't have the significancedifference, the Feeding-Formononetin xanthate-group rats kidney tissue Bax, Bax/Bcl-2are significance to be lower than the Feeding-Trifolium Pretense L-group(P＜0.01, P＜0.05).Conclusion:1. Aerobics have certain protective function to the kidney damage which creates bythe hyperlipemia, can reduce the rats' kidney tissue shape structure change which thehyperlipemia creates, and suppresses some. promotion kidney fibrosis cell factor therelease.2. Formononetin xanthate and Trifolium Pretense L belong to the flavonoidcompound, have certain function to fall the blood fats, can reduce the rats kidney tissueshape structure change which the hyperlipemia creates, and can suppress the kidney tissue to damage in the process some inflammation factor, the cell growth factor release.Among them, in suppressing the expression of kidney tissue cell factor VEGF, TGF-β, CTGF, NF-κB and a-SMA, Feeding-Trifolium Pretense L-group effect significancesurpasses the Feeding-Formononetin xanthate-group(P＜0.01), but the Formononetinxanthate-group rats suppress kidney tissue cell perishes the effect to surpass theTrifolium Pretense L-group weakly, two groups in suppressing the expression of kidneytissue cell factor ICAM, VCAM, MCP-1, OPN, Desmin and vimentin don't havethe significance difference.3. Formononetin xanthate+aerobics and Trifolium Pretense L+ aerobics to protectthe kidney damage effect which the hyperlipemia creates to be most obvious, surpasspure aerobics and pure medicine group.Prompts two kind of flavonoid compound as well as aerobics treatment havecertain protective function to the rats lipin kidney harm. Possible because the flavonoidcompound as well as has aerobics be able to fall the blood fats, and through reduces thelipin immersion and the peroxidization as well as the inflammation cell infiltration andso on, thus the suppression renal glomerulus membrane cell, the endothelial cell as wellas the extracellular matrix multiplication and the tubulus renalis cell phenotypetransformation.