The Biological Researches On The Remodeling Of Movement To The High Fat Diet Rats' Intermyocardial Collagen Network

Purpose: Observe the Remodeling of movement to the high fat dietrats' Intermyocardial Collagen Network and discuss the Mechanism.Method: Author used 50 male SD rats in 3 months age (provided by Xi'anJiaoTong University Medical school animal administrative center) and the weight were220±20g. Then divided them into 7 groups stochastically: the normal control group (Agroup), the aerobic training group (B group), the fatigue movement group (C group), thehigh fat control group (D group), the high fat with movement group (E group). A, B andC groups used the national standards rodent animal' s dry feed, while D, E, F and Ggroups used the high fat feed. During the experimental period all rats had free diet in thefeeding room with 17℃-23℃temperature and 40％-60％humidity. The test startedafter the adaptability feed for a week and the movement training was in the way thatincreasing run intensity on the running platform. The initial velocity was 15m/min andthe time was 20 minutes. The physiological load of exercise referred to Bedford' sresearch. Starts the speed is 15m/min. A and D groups did not attend the training.Aerobic training was 5 days each week. The speed increased by degrees was 3m/minand the time was 5 minutes. The max speed was 20m/min. Time runs slope for 60minutes, for 5°. Fatigue training was 6 days a week. The increasing speed was 3m/minand the time was 5 minutes. The max speed was 35m/min. Time runs slope for 60±10minutes, for 10°.The entire training process continued 8 weeks, which used electricalstimulation to force the rats to run (electricity＜1mA). E group did not attend thetraining until the 5th week with aerobic training. The physiological load of exercise was5 days each week. The increasing speed was 3m/min and the time was 5 minutes. Themax speed was 20m/min and the max time was 60 minutes. All the training processcontinued for 4 weeks and finished in the 8th week. Then the author selected theexperimental samplings separately, including the Heart function testing, the blood, thelight microscope, the electron microscope and the immunohistochemistry specimen.Result:1. Movement has produced effect to rat heart function. The aerobic training madethe HR,LVEDP drop, LVSP,±dp/dtmax和±d~2p/dt~2max rise, indicate the heart contracting an ability strengthening. The fatigue training made the HR,LVEDP rise,LVSP,±dp/dtmax和±d~2p/dt~2 max drop, indicate the heart contracting an ability comingdown. The Hyperlipidemia made the HR,LVEDP Rise by a wide margin, LVSP,±dp/dtmax and±d~2p/dt~2 max notable lessening, the contraction ability indicating theheart accepts effect. The high fat with movement made HR drop, LVSP,±dp/dtmaxincrease,±d~2p/dt~2 max Increase by obviously. Indicate the heart function strengthening.2. In having oxygen to train but using rat circulation blood, AngⅡnotable reduces,Cardiac muscle hydroxyl praline and ALD reduce obviously. The fatigue training madeAngⅡin blood, Cardiac muscle hydroxyl praline and ALD notable power-up. This andHyperlipidemia Identical. Every index comes down to some extent without exceptionthe high fat with movement group. Indicate motion training to high fat rat cardiacmuscle collagen weight mould AngⅡand ALD go hand in hand with blood plasma.3. Rat cardiac muscle mesenchyme collagen network weight moulds and movesthe osculation training relation. Have oxygen to move but make rat cardiac muscle fibrerude increasing, cardiac muscle mesenchyme collagen hyperplasia, to adapt to enhancedneed of loose cardiac muscle cell contraction force, ensure that cardiac muscle thins outthe force delivery, cardiac muscle contracts a function strengthening thereby. Wearymotion may lead to the rat cardiac muscle Shu room, cardiac muscle cell group and cellroom large amount of no proportional hyperplasia of collagen fiber, function havingdamaged the heart's lead to heart rigid degree increase by, conform to nature lessening,direct. Explain that having oxygen to train has changed the rat cardiac musclemesenchyme collagen, has made the heart function strengthen. High grease meals haslost bast and the cardiac muscle cell, cardiac muscle has thinned out excessivehyperplasias of quality collagen, The hyperplasia collagen is being bound uphypertrophic cardiac muscle, "has closed" the space that the cardiac muscle cellcontracts, function having increased rigid degree of the cardiac muscle, havingdamaged the heart's. By having oxygen to train, using the high grease rat cardiacmuscle mesenchyme collagen, degradation has reinforced, strengthened heart'sfunction.4. Notable becoming fagged moving expressing of rat cardiac muscle TNF-a eggwhite has raised, TNF-a has participated in in explaining that rat cardiac muscleorganizes under weary motion cardiac muscle loss and weight structure. High greasemeals rat cardiac muscle organizes middle TNF-a expressing the nature work nature power-up, the person expresses lessening by that motion interferes with the queen,shows moving the function training expressing being able to cut down cardiac muscleorganization middle scorching factor TNF-a, being able to improve the heart.Organization is hit by the MMPs activity, therefore, the weight thinking that motionarouses the high grease meals rat cardiac muscle mesenchyme collagen, moulds andTNF-a participates in MMPs since TNF-a is able to improve cardiac muscle expressrelevant.5. But have oxygen to move rat cardiac muscle AT-1R's expressing notablelessening, notable becoming fagged moving rat cardiac muscle AT-1R expressing rises;High grease meals rat cardiac muscle AT-1R expressing is notable rise, reduce by thatthe person expresses notable after motion interferes with. Since AngⅡcan stimulatecardiac muscle becomes fibre proliferation of cells, collagen combining and the basematerial collagen deposit, guides cardiac muscle to fibre-rization. Therefore, haveoxygen motion but notable to reduce rat cardiac muscle organization AT-1R expressing,restrain cardiac muscle's thereby fibre-rization, improve the contraction of the cardiacmuscle function.6. Notable becoming fagged moving rat cardiac muscle MMP-13 expressing rises.High grease meals rat cardiac muscle organizes middle, MMP-13 expressing thenotable power-up, express by that motion interfering with the queen but notablereducing the person, expressing having oxygen to move rat cardiac muscle MMP-13 isnot notable but, the hyperplasia explaining that high grease meals is able to lead to thecardiac muscle collagen fiber, has oxygen to move the structure being able to improvecardiac muscle, expressing restraining or cutting down MMP-13, the hyperplasiacutting down a collagen fiber thereby.7. Notable having oxygen to move and becoming fagged moving rat cardiacmuscle organization middle NOS expressing raises, and has some set notable difference.High grease meals rat cardiac muscle is organized being hit by NOS's expressing thenotable power-up, motion interferes with the queen but notable reduces the personexpress. Again, since the NOS vigour and the contents power-ups lack for blood withcardiac muscle the hyperplasia pouring loss and the collagen concerns an osculation,NO can restrain hyperplasia of the collagen fiber. Therefore, move the cause trainingexpressing being able to reduce high grease meals rat cardiac muscle organizationmiddle NOS to may be to restrain whose collagen fiber hyperplasia. Conclusion:Have oxygen to move the function being able to strengthen the heart, messengercardiac muscle mesenchyme collagen network benign weight moulds, can restraincardiac muscle aroused by high grease meals fibre-rization, and have a closerelationship with cardiac muscle TNF-a, AT-1R, NOS and the MMP-13 expressing.