Histology Effects Of Little Needle-scalpel On Treating Muscle Injury

Muscle injury is a kind of sports injury with high incidence rate, and it has a bad effect on the players' motor ability. We are short of better therapy in clinical treatment. Recently, with the investigation of little needle-scalpel, we found that it can remit adhesions caused by muscle injuries and accelerate the reversion of motor ability. But all of these investigations are clinical studies, most of them are subjective and short of microcosmic experiment. It limits the development of little needle-scalpel. Our experiment is to investigate the muscular histology changes, and offer basis for little needle-scalpel treatment. In this way we can promote its development.We divide 20 Japanese rabbits into four groups: two rabbits made up the Natural Control Group (N10), observed the normal percentage of muscle fiber. The rest are injurymodels (not completely cut). After ten days choose six at random (Model Group, M10). The left legs are spontaneous recovery (SR), and the right legs are treated by little needle-scalpel (T), divide intoSR20, SR28, T20, T28. Make paraffin sectioning to observe histology changes. First, take photos of each leg's sections, then use 10X10 net shelf to count the area of muscle fiber, at last disposal these data by SPSS 10.0. Conclusion was made as following:Naked eye observations:l.Ten days later: Fasciae adhere skin, and have some fatty substance. The surface is rough, and has some blocks. The injury place is white, has some mucus. It feels rough. It shows we can duplicate gastrocnemius muscle injury using not completely cut.2.Twenty days later: The spontaneous recovery group(SR): Fasciae still have some adhesions with skin, and some fat tissue. The injury place is easy to be found with naked eyes. The treated by little needle-scalpel group(T):There is no obvious adhesion, no fatty substance, and no mucus. The surface is smooth. It is difficult to find the injury place without the black suture silk.3.Twenty-eight days later: SR: The adhesion became little, but still has some fatty substance. T: The colour of the injured muscle is normal, and has no hyperplasia substance. The surface is smooth, and the injured muscle can be seen with the dissection mirror.Microscopy observations:1. T20 compared with SR20, the difference is significant (P<0.01). It indicates little needle-scalpel treatment can accelerate the recovery. At the same time, the T20 tissue sections show muscle fibers line up in order, sarcomere, light band and dark band are clear. The newly born muscle fibers are more maturity.2. T28 compared with SR28, the difference is significant (P<0.01). The T28 is close to normal.3. T20 compared with SR28, the difference is notsignificant (P>0.05 ) . It indicates little needle-scalpel treatment can accelerate the recovery, and make the newly born muscle fibers more maturity.4. T28 compared with SR28, the difference is significant (P<0.01). It indicates the injury is too severe to recover in 28days.