The Differentially Expressive Research On The Proteome Of Ventricular Muscle On Fatigue Rat Induced By Exercise

This study established the differentially expressive imagefilms of the ventricular muscle on the control group andexercise group which has taken a long-term progressive loadvolume training by adopting proteomics skills, to seek themeaningful proteins of ventricular muscle which response toexercise stress, and to probe into the law that inducedmyocardial fatigue from the level of quantitative andqualitative proteome.As research model, the male SD rat of control group andexercise group executed after anesthetize at the same time 6hours rest later after seven week progressive increasing loadvolume training of high intensity, and the total protein ofventricular muscle was extracted, and then separated by 2D-PAGE,the gel stained by Coomassie brilliant blue G-250, and theanalysis of image films of gel and data acquisition was doneby PD-Quest software. The objective protein spots whichdisappeared and the differential expressive volume increasedmore than 5-fold after exercise were select for mass spectraanalysis. The experimental results show that: the per match rate ofcontrol group and the exercise group respectively is 79％±10.46％and 77.1％±8.23％, the per coefficient correlation is0.73 which demonstrated that the image films with higherreproducibility was attained. The protein spots amount ofcontrol group and exercise group respectively were 352±17 and338±21. There are 99 protein spots that differently expressedin which five protein spots disappeared, 12 were respectivelyupgraded and downgraded more than S-fold, 52 protein spotsupgraded and 42 downgraded more than 2-fold after exercise. 7proteins were identified in 9 differentially expressed proteinspots. Long-chain specific acyl-CoA dehydrogenase,NADH-Ubiquinone Oxidoreductase 75KD subunit,stress-70 protein,Tropomyosin-1 alpha chain were disappeared after exercise,Nitrilase family, member 2 was upgraded, and a 21KD unknownprotein and myosin-6 were downgraded.The ventricular muscle proteome remarkably changed afterexercise. The protein spots disappeared and downgraded afterexercise have strong relationship with disturbance of musclecontraction regulation and metabolic style turn and stressreaction. This demonstrated that the happens of fatigue inducedby long-term progressive load volume training was related with the decrease of muscle contraction capacity, the difficultyin energy supply and the damage to the cardiac cells. It isnoteworthy that the objective proteins which werecharacteristic of exercise stress and have not studied insports medicine area like Long-chain specific acyl-CoAdehydrogenase, stress-70 protein and so on were screened. Thisprovide some new thought and settled prophase base for furtherresearching fatigue induced by exercise.