Expression And Significance Of TGF-β1 TGFβRⅠ And C-myc In Lymphadenoma Cell

Objetive:Lymphoma is a kind of tumer of lymphoid stem cell prosoplasis and clonal proliferation.It is one of the common cancer in pediatrics with an increasing incidence,whose etiopathogensis is unknown.At present, traditional chemotherapy is still the main method of therapy,but the drug resistance and side effect of chemotherapy is one of the important factors to influence long term survival.Therefore,it is significant that searching for etiological factor and exploring a more effective prevention and cure measure to improve the patient's prognosis and quality of life.TGF-β( transforming growth factor beta) is a multipurpose polypeptide growth factor. Nearly every internal cell all proudects TGF-βand its'receptor.For the past few years,it discovered that TGF-βhas very important accommodactal contribution for the growth differentiate and immune fuction of cell.TGF-βcorrelates with the genesis and development of tumor.In the process of the mutistance of tumorigenesis,TGF-β1 has biphasis effect.At the morning of tumorgenesis,it inhibits tumour cell to growth,TGF-β1 makes cells'growth cycle stop G1 stage through its'signal passageway,and then inhibits cell multiplication and derivns cell differentiation or apoptosis.But at the middle and later stage, TGF-β1 is possible to precipitate tumour cell to display malignant characteristics and at the time of cell aggraration,the moity of TGF-β1 changes.Multi-genetic mutution's cell tolerate to TGF-β1,which makes cell escape growth inhibiting that mediates by TGF-β1.At last,cell growth is out of control,derivns cell mutation and promotes tumour to make advancement.Generally speaking, TGF-βsignal passageway which is a tumor inhibiting acess is contruction by ligand,acceptor,Smad proteinum and target gene of transcription regulation.The dysfunction of any element in the passage way all can make the disorder of transduction,which influences the cytobiology effect of TGF-β.C-myc,as a transcription factor,has important effect at accommodating cell's proliferation,differentiation and apoptosis.For the past few years,it discovered that TGF-βexpress higher in many tumors and its'receptor expressed anrmaly.But at present,the study in the espression of TGF-βand its'receptor in lymphoma is very few at home and abroad.In this study,in order to investigate that the interaction of TGF-β, TGFβRⅠ(transforming growth factor beta receptorⅠ, TGFβRⅠ)and c-myc in lymphoma's genesis and development,we adopted the paraffin block of children's lymphoma as sample and used flow cytometry to detect the expression of TGF-β, TGFβRⅠand c-myc.Methods: Assembleed 30 exemple lymphaden of paraffin inbedding sample of lymphoma in The Fourth Hospital of Hebei Medical College from 2006 year to 2008 year,10 exemple lymphaden of paraffin inbedding sample of reactive hyperplasia as normal control.First used HE staining to make a definite diagnosis,then used Flow Cytomety(FCM) to detect the expression of TGF-β, TGFβRⅠand c-myc.Results:1 FCM showed:The expression of TGF-βin lymphoma were 1.400±0.250;the expression of TGF-βin reactive hyperplasia were 1.000±0.06.Compared to control group, The expression of TGF-βin lymphoma exited variability(P<0.05).Through independent-samples T test, the expression of TGF-βin lymphoma were obviously higher to control group(P<0.05).But the expression of TGF-βin T cell origin lymphoma compared to in B cell origin lymphoma had no variability(P>0.05).2 FCM showed: The expression of TGFβRⅠin lymphoma were 1.377±0.213; The expression of TGFβRⅠin reactive hyperplasia were 1.000±0.109.Compared to control group, The expression of TGFβRⅠin lymphoma exited variability(P<0.05).Through independent-samples T test, the expression of TβRⅠin lymphoma were obviously higher to control group(P<0.05).3 FCM showed: The expression of c-myc in lymphoma were 1.287±0.209, The expression of c-myc in reactive hyperplasia 1.000±0.019,Compared to control group, The expression of c-myc in lymphoma exited variability(P<0.05).Through independent-samples T test, the expression of c-myc in lymphoma were obviously higher to control group(P<0.05).Conclusions:1 The expression of TGF-β1 in lymphoma were obviously higher to control group.It was explanation that TGF-β1 had very important contribution in the process of lymphoma's genesis and development. But the expression of TGF-β1 in T cell origin lymphoma compared to in B cell origin lymphoma had no variability.It was explanation that TGF-β1 possiblely had same contribution in the process of genesis and development of T cell origin lymphoma and B cell origin lymphoma.2 The expression of TGFβRⅠin lymphoma were obviously higher to control group.It was different to the much study of epithelium source tumor.But it is similar to the study of leukocythemia. It was explanation that it has different pathogenesy between hematopathy and entity tumor.The overexpression of TGFβRⅠhad very important contribution in the process of lymphoma's genesis and development too.3 Between the expression of TGF-β1 and TGFβRⅠhad orthodependability. We inferred that this orthodependability has relation with the mechanism of the compounds of TGF-β1 and its'acceptor and the degenerative feedback mechanism in the TGF-β1 signal passageway.4 The expression of c-myc in lymphoma were obviously higher to control group.We inferred that c-myc proteinum promoted cell from G0/G1 stage to S stage through up-regulation the associated protein of cell cycle ,which mad the malignant proliferate of lymphoma cell and mad lymphoma celle escape the contribution of antiproliferate and promoting apoposis.5 Between the expression of TGF-β1 and c-myc had orthodependability. C-myc is a important targent gene of the TGF-βsignal passageway's downstream. It explained that becauseed of the TGF-βsinal passageway signal transduction was dysfunction,which mad c-myc who was a proto-oncogene express higher because missing negative control of TGF-β1 who was a restrain-cancer factor.