Effects Of Excessive Fluoride On The Expression Of Some Non-collagenous Proteins And Transforming Growth Factor β

The bone damage of chronological fluorosis relates to multi-cells and extracellular matrix, which participate in bone transition.Non-collagenous proteins,as the important ingredient of extracellular matrix,play an important role in the osteogenesis and bone transition.But the systematical and further research on the influence of related fluorides to non-collagenous bone matrix proteins and the function of proteins of transformation of bone matrix to the development of fluorosis of bone,were rarely done.Via fluoride concentration in drinking water to replicate casts of white-rat fluorosis and culture in vitro osteoblast fluoridized,and adopting the technology of morphology, immunohistochemistry,in situ hybridization,RT-PCR,Western blot and ELISA,this experiment mainly carried out the research on the influence of fluoride to the following non-collagenous proteins,such as osteocalcin,osteopontin,osteonetin,as well as the expression of transforming growth factorβwhich closely relates to them.By observing the bone tissue casts of fluorotic rats, bony trobecula of metaphysis crowded together;osteoblast and osteoclast increase tremendously,and...