Association Of Serum Vaspin Concentration With Insulin Resistance And Inflammatory Mediators

Objecfive: To investigate the change of serum vaspin levels in patients with obesity and newly diagnosed type 2 diabetes mellitus (T2DM) , and the association of serum vaspin levels with anthropometric parameters, glucose-lipid metabolism parameters, insulin sensitivity index, and inflammatory factors, in order to explore the relationship between vaspin and insulin resistance, inflammation, as well as provide some theoretical foundation for the occurrence of obesity and T2DM.Methods: 90 patients with newly diagnosed T2DM and 60 subjects with normal glucose tolerance (NGT) were selected and each group was divided into obese (OB) and normal weight (NW) subgroups according to body mass index (BMI)≥and < 25kg/m2.The level of fasting serum vaspin, TNF-αand IL-6 were measured by enzyme-linked immunosorbent assay.Meanwhile, fasting plasma glucose (FPG), fasting insulin (FINS), Hemoglobin A1c (HbAlc) and lipid profiles were also measured.BMI,body fat percentage (BF%) and waist-to-hip ratio (WHR) were evaluated and insulin sensitivity was assessed by HOMA-IR and HOMA-β.Results:①The serum vaspin concentration in obese group was significantly higher than in normal group (P<0.001), but there was no difference between T2DM and NGT groups (P>0.05).②The serum TNF-αand IL-6 concentrations in T2DM group were significantly higher than in NGT group (P<0.001). Meanwhile, either T2DM group or NGT group, subjects with OB had higher serum vaspin levels than those with NW (P<0.05).③Pearson correlation analysis demonstrated that serum vaspin levels were positively associated with sex, BMI, BF%, W, WHR, FINS, TG and ln (HOMA-IR) (r=0.278, 0.431, 0.455, 0.274, 0.319, 0.266, 0.189, 0.163, P<0.05, respectively), but negtively associated with HDL-C, ln (TNF-α) and ln (IL-6) (r=-0.196, -0.169, -0.222, P<0.05, respectively).④In a multiple stepwise linear regression analysis, BF%, IL-6, WHR, BMI, TNF-αand HOMA-IR were independent determinants for serum vaspin concentrations.Conclusion:①Serum vaspin concentrations were significantly higher in subjects with obesity.②Vaspin was strongly associated with obesity and insulin resistance. Monitoring the serum vaspin levels may have a a very important value for understanding the degree of obesity and insulin resistance.③Vaspin may have the effect of inhibiting inflammation. It may provide another new breakthrough for the development of novel diabetes drugs.