Expression Of EPOR In Prostate Cancer Cell PC3 Treated With The Erythropoietin

Objective:To investigate the influence of erythropoietin on a selective the erythropoietin receptor in human prostate cancer PC3 cells.Methods:Prostate cancer PC3 cells (2×10~4/ml)was cultured with EPO of different concentration(0～50 IU/ml)for 3h,6h,12h,24h,48h in vitro and effects of EPO on the proliferation in PC3 cells was measured by CCK-8 assay; After the PC3 cell being cultured with EPO for 48h,its morphological change was analyzed by optical microsopy; The protein expressions of EPOR affected by EPO was determined by immunocytochemistey.Results:PC3 cells were incubated in MEM in vitro and passaged for 2～3 days.CCK8 colorimetric method showed that EPO could promote the proliferation of PC3 cells in a dose and time depent manner.The best concentration of EPO which promoted PC3 cells was 50 IU/ml,and the best time was 48h. Typical morphological change could be observed by using normal microscope after treatment with EPO; The expression level of EPOR increased gradually with the increase of EPO concentration by the way of immunocytochemistey. (P<0.05)Conclusion:EPO can significant growth effect in PC3 cells and the inhibiting apoptosis in PC3 cells could be induced by EPO in this study.The increase of EPO concentration may be related to the up regulation of the expression of EPOR.