The Experimental And Clinical Study Of The Relations Between Dynamic Change Of AP And Brain Damage After Seizures Neurology Hang Yan Directed By Zhang Hui

Objective To observe the dynamic change of plasma AP after seizure from animal experiments and clinic study. It would provide the theoretical evidences for that AP could be one of the early sensitive biochemical markers in seizure-induced brain damage. Methods 1. The animal experiments: Made comprehensive attacks model of mouse (Maximal electroshork seizure test) and partial seizures model ( Metrozol Seizure test ) according to the completely random principle, then collected blood after seizure at different time points (3h, 6h, 12h ,24h, 48h and 72h) to determine the level of plasma AP. Then to observe the dynamic change of plasma AP in epileptic group, whether it had statistical significance to compare with the matched control group or not. 2. Clinical aspects: 62 patients with idiopathic epilepsy were chose from our neurology outpatient clinic, collecting relevant information and monitoring the dynamic electroencephalogram (EEG) was done in 24hrs, then the plasma AP level was determined at the same time. 46 healthy adults and children were chose as control group matched with age and sex to epileptic group. Observed the dynamic change of plasma AP at different time points (1d, 2d, 3d) and analyzed duration, the times of seizure, attack frequency, medication, the time of collecting blood after seizure, epileptiform discharge site and extend in electroencephalogram (EEG), whether they was related to plasma AP after seizure or not. Results 1.The animal experiments: The level of plasma AP in the epilepsy mouse(3.33±0.96)was significantly higher than the normal control group(0.93±0.12)(P<0.05);There was no statistical significance in either comprehensive attacks or partial seizures of plasma AP; The level of plasma AP in the epilepsy mouse had the significantly rising trend at 3 hours after seizure and reached the peak at 24 hours, then gradually declined at 48 hours and 72 hours. 2.Clinical aspects: The level of plasma AP had statistical significance between epilepsy group(5.60±1.22)and normal control group(1.99±0.66). The level of plasma AP in the epilepsy group increased within 24 hours after seizure, and reached the highest point at 24 hours, then gradually decreased on the 2nd day and 3rd day; The level of plasma AP was positively correlated with the times of seizure, attack frequency and negatively correlated with medication and the time of collecting blood after seizure, but not with duration, epileptiform discharge site and extend in electroencephalogram (EEG). Conclusion The level of plasma AP in the epilepsy group was significantly higher than the normal control group in both animal experimental and clinical study. And the dynamic change of plasma AP after seizure in animal experiments was similar to that of clinic study. It suggested that the plasma AP might be as the early indicator of seizure-induced brain damage and the predictor in the early stage of brian damage caused by seizures. The times of seizure, attack frequency, medication and the time of collecting blood after seizure were the effective factors of the AP level, indicated that AP may be related to the degree of brain damage and drug protective function.