Expression Of The KLF5 Protein In Renal Cell Carcinoma And Its Significance

Objective: renal cell carcinoma is a common urological malignancy, accounting for 3% of whole malignancy , the incidence of urinary tract tumors is after bladder cancer in our country, the second highest, and the incidence is increasing year by year. The progress and transfer of renal cell carcinoma is the main causes of death in patient's. Therefore, to investigate the mechanisms of transfer and the progress of renal cell carcinoma for improving the therapeutic effect, raising the survival rate is very important. Krüppel-like factors (KLFs) belong to the transcription factor family containing zinc-finger, Krüppel-like factor 5 (KLF5, also known as BTEB2/IKLF)as,as a member of the family,mainly involved in regulating cell proliferation and differentiation. Its expression in renal cell carcinoma, especially relations of the occurrence and the development with renal cell carcinoma, has not been reported. This study explored the expression of KLF5 in renal cell carcinoma and its clinical significance.Method:1 KLF5 gene expression of 51 cases of renal cell carcinoma tissues and 20 cases of normal renal tissues were detected from the protein levels by Immunohistochemistry SP method.2 Western blotting analysis: Equal amounts of protein extracts from normal and renal cell carcinoma tissues were separated on 8% SDS-PAGE, and then blotted onto PVDF membrane. The membrane was immunological stained with specific antibodies. The results were analyzed by digital imaging system.Results:1 Immunohistochemistry results showed that: KLF5 were expressed in renal cell carcinoma and adjacent normal tissues. In carcinoma tissues, the expression of KLF5 was mostly diffuse, loss of polar expression, staining levels increased, and most expressed in the membrane and the cytoplasm; but, in adjacent normal tissues, the expression are only in the distal tubule,collecting duct cell membrane and cytoplasm, moderate staining intensity, and the climax was distributed. The positive rates of KLF5 in renal cell carcinoma and normal renal tissues were significantly different (P=0.000), renal cell carcinoma was significantly higher than in normal tissues. The expression of KLF5 rate was no correlation with histological type (P>0.05), but the expression of renal clear cell carcinoma and non-clear cell carcinoma was significantly different, the former were higher than the latter, and no correlation with grade (P> 0.05).In addition, the increased KLF5 is positively correlated with clinical stage (P<0.05).The level of KLF5 in renal carcinoma tissues with metastasis was higher than no metastasis, so the difference was statistically significant (P <0.05).2 Western blotting analysis showed that, the levels of KLF5 protein in tumor tissue was significantly higher than normal tissue, the difference was statistically significant (P <0.05).Conclusion:1 The levels of KLF5 protein in renal cell carcinoma tissue were higher than normal tissue.2 The levels of KLF5 protein in renal cell carcinoma had no correlation with the histological typ.3 The levels of KLF5 protein in renal cell carcinoma had no correlation with the Fuhrman's grade of renal cell carcinoma.4 The levels of KLF5 protein in renal cell carcinoma were positively correlated with Robson's clinical stage and metastasis of renal cell carcinoma.5 The overexpression of KLF5 protein may be a important biological marker for the invasion and metastasis of renal cell carcinoma, so detecting the expression of KLF5 is important to assessing the metastasis and prognosis of renal cell carcinoma .