| Objective:To investigate the expression and correlation of Inhibitor of DNA binding/or differentiation-4 (Id4) and Transcription factor E2F1(E2F1)in the renal clear cell carcinoma(RCCC), and to explore the role of them in the pathogenesis of RCCC. we expected that the study will help us to elucidate whether the two involved in pathologic grading of cancer and clinical stage, and to provide novel therapeutic targets for clinical treatment of renal cell carcinoma(RCC).Methods:The expression of Id4 and E2F1 were detected with immunohistochemical SABC methods in 40 cases of RCCC,10 cases of adjacent tissues and 10 cases of normal renal tissues. The statistical analysis methods of data:the count materials were evaluated withχ2 test or Fisher probabilities in 2x2 table; the grade materials were evaluated with nonparametric rank sum test (Mann-Whitney U test or Kruskal-Wallis Htest);and the correlation using Spearman rank correlation analysis.Results:(1). The expression of Id4 and E2F1 in the RCCC were Significantly higher than adjacent tissues and normal tissues. The positive rate of Id4 in RCCC group was 67.5%(27/40), compared with the adjacent group 30%(3/10) and normal group 20% (2/10) was significantly higher(P<0.05). Moreover, the positive rate of E2F1 in the there groups were 75%(30/40)in the RCCC group,40%(4/10) in the adjacent group and 20%(2/10) in the normal group. Both of the positive rates of Id4 and E2F1 in the RCCC group compared with the other two groups were significantly different (P<0.05), while the adjacent group and the control group have no significant difference between the two groups (P> 0.05).(2). There is no significant correlation between the expression of Id4,E2F1 and the pathologic grading of cancer. The positive rate of Id4 was increased with higher pathological grade, that was gradeⅠ58.3%(7/12),gradeⅡ70%(7/10,gradeⅢ70%(7/10)and grade IV75%(6/8), but there are no significant correlation between them (P> 0.05). At the same time, the positive expression rate of E2F1 were grade I 66.7%(8/12),gradeⅡ80%(8/10),gradeⅢ80%(8/10)and gradeⅣ75%(6/8), there are also no significant difference between the four groups (P> 0.05).(3). In the prophase renal cell carcinoma (Ⅰ-Ⅱphase),the positive rates of Id4,E2F1 were 65.5%(19/29) and 72.4%(21/29). At the same time, the positive rates of them were 72.7%(8/11)and 81.8%(9/11) in the advanced stage (Ⅲ-Ⅳphase). The expression of them were increased with higher clinical TNM staging(cTNM), but it is Statistically insignificant (P> 0.05).(4). In the clear renal cell carcinoma, the expressions of Id4, E2F1, that was common negative in 5 cases, while positive expression in 11 cases, and strong positive expression in 9 patients.Another 15 patients have a different expression. Spearman correlation analysis showed that both of them existed a positive correlation in the clear cell renal carcinoma(r=0.588, P<0.05).Conclusions:High expression of Id4 an E2F1 play an important role in pathogenesis of RCCC. And both of the expressions of them has a positive correlation, maybe have a synergetic effect to promote tumorigenesis,or promote tumorigenesis alone. However, there is no significant correlation between the high expression and tumor grade, clinical stage. It is less value for the evaluation of tumor prognostic in patients. Id4 and E2F1, which may be provide a new option for renal carcinoma targeted therapy, are attractive targets for antitumor therapy. |
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