Intervention Study Of Yunnan Baiyao On Experimental Rheumatoid Arthritis And Periodontitis Correlation

Objective:The purpose of this study was to discuss the relationship between periodontitis and rheumatoid arthritis, the mechanism of Yunnan Baiyao controlling inflammation and decreasing bone damageMethods:128 male SD rats,7～8 week-old, were randomly divided into four groups: normal group (N,32rats), simple model group (SM,32rats), model with Yunnan Baiyao (YM,32rats), model with methotrexate group (MM,32rats). each rat,except normal group,were injected 0.2ml collagen emulsions in tail root and 0.1ml collagen emulsions in the metatarsal mat of the left foot, on the day of 7 th and 21th after the first injection,reinjected 0.2 ml collagen emulsions to each rat. On the day of 7 th,did the gastric perfusion with Yunnan Baiyao and methotrexate to each rat of model with Yunnan Baiyao and metatarsal. On the week of 1th,2th,3th,4th,blood sample was taked from heart under anesthesia and tissues were collected from each group.Observe indicators include:(1):rat weight, foot claw thickness, arthritis score. (2):histologic index:HE staining was emplayed to observe the changes of alveolar and gum tissue and infiltrating joints of inflammatory cells.The TRAP staining was emplayed to observe the osteoclast in joint. With Alkaline phosphatase and RANKL immunohistochemical staining to study positive changes of express cell in alveolar bone. (3):serological index: ELISA was used to detect the level of the IL-1beta, PGE2 in serum.Statistical analysis were performed with pairwise comparison of the differences sample mean.Results:The joint inflammatory response in group YM and group MM was reduced along with the extension of time of medication administration, the level of IL-1 beta, PGE2 expression was significantly inhibited(Table6-7). Form the second week with the medication,the inflammation response was less in group YM and group MM compared with group SM(Table2-3). Weight growth,in the first 12 days, were lighter than normal group (Table 1).positive osteoclas were only obsered in group SM in 4w. Compared with group SM after did medicine 1 w, the express of RANKL positive in alveolar bone obviously decrease in group YM and group MM(Table4), alkaline phosphatase positive express area had increased in group YM and group MM after 3w with medications(Table5).Conclusion:Rat experimental rheumatoid arthritis can potentially raise the express of activator of the osteoclast(RANKL), compensately promote bone active factor (ALP) expression. Yunnanbaiyao and methotrexate can restrain the expression of the osteoclast active factor, promote osteogenesis active factor expression. Meanwhile, Yunnan Baiyao, like methotrexate, can reduce the joint swelling degree, inhibit the development of the joint inflammation, reduce the level of PGE2 and IL-lbeta in the peripheral blood plasma.