Anti-inflammation Effects Of Yunnan Baiyao On Experimental Periodontitis And Experimental Diabetes Mellitus Periodontitis

Objective:To evaluate the anti-inflammation effects of Yunnan Baiyao on experimental periodontitis and experimental diabetes periodontitis,and explore it's possible in vitro mechanism of the inflammatory response.Methods:Seventy-five 6-week-old healthy male SD rats were involed in this study, the experimental animals were randomly divided into three groups:control group(N group=15rats),diabetic periodontitis group(DP group=30rats),periodontitis without diabetes group(P group = 30rats).DP group was injected STZ to induce diabetes model,after then both DP group and P group were silk ligatied to establish the periodontitis model.When model was established,periodontitis rats were randomly divided into experimental group(P1 group,n=15rats) and the periodontitis control group(P2 group,n=15rats);DP group rats were randomly divided into experimental diabetic periodontitis(DP1 group,n=15rats),and diabetic periodontitis control group (DP2 group,n=15rats).Rats in P1 group and DP1 group,Yunnan Baiyao 1ml (50mg/ml) were gavaged daily respectively rats,and the rats were gavaged 1ml of distilled water per day in P2 group and DP2 group.Taking the blood sample from the heart for test TNF-α,IL-1 and AGEs.After the rats dead,taking the maxilla bone sample with 4%paraformaldehyde,and the sample will be used to test TNF-αby immunohistochemistry.Observed indicators include:(1) clinical parameters:probing depth(PD),alveolar bone loss(ABL);(2) histological indicators:measure and analysis TNF-αoptical density in periodontal tissue;(3) Serum IL-1βand TNF-αlevel changes were analysis by ELISA.(4) The determination of serum AGEs.Data using single-factor analysis of variance statistically.Results:Experimental periodontitis and experimental diabetes periodontitis SD rats gastric lavage periodontitis(including Yunnan Baiyao 50mg/ml) can reduce the periodontal inflammation.In 4wk and 6wk,the content of TNF-αand IL-1βin rats serum,the content of TNF-αalveolar bone,periodontal probing depth of appointment all express:P1group＜P2 group,DP1 group＜DP2 group.In 6wk,alveolar bone loss P1 group＜DP1 group,P2 group＜DP2group,and the content of AGEs in serum DP1 group＜DP2 group.Conclusions:Yunnan Baiyao can inhibite inflammation response of animal experimental periodontitis and experimental diabetes periodontitis,and also can reduce the destruction of periodontal tissue.the Chinese medicine also have same function to inhibite TNF-αexpression in alveolar bone,at the same time,it reduce the content of serum TNF-αand IL-1β.We also know it's possibility inhibition function for the serum AGEs.