The Related Study Of Immune-inflammation In The Progression Of Benign Prostatic Hyperplasia

Background and Objective:BPH (Benign Prostate Hyperplasia) is a common disorder introducing low urinary tract symptomes of the aged male population. An estimated 42% of men 51 to 60 years of age have histological BPH. The incidence increases to over 70% in men 61 to 70 years of age and to almost 90% in those 81 to 90 years of age. With related of social structure aging, the incidence of BPH in our country become higher.But but no theory show the dependent role in the pathogenesis of BPH. In the last years histology inflammation appears to be potentially important in the progression of BPH. In this researchment, immunohistochemical analysis was used to examine the expression of CD8, CD20, Interleukin-6, Interleukin-23 and TGF-βin BPH tissues. Main clinical data were compared and analyzed between groups of variant expression results.We intended to investigate the effect immune-inflammation in the development and progression of BPH.Materials and Method:A totally of 60 patients of BPH from department of urology, second hospital of Tianjin medical university undergoing surgical resection or TURP treatment, with accession from July 2009 to September 2010, were included in this study. The average age is 70 with a range of 46～85 years old that are confirmed simple benign prostatic hyperplasia by HE staining in pathology department.Strept Actividin-Biotin Complex (SABC) method was used to detect the expression of CD8, CD20, IL-6, IL-23 and TGF-βin BPH tissues.Staining steps were carried out according to kit instructions, the results were observed by light microscope, and buffy particles in cells were considered as positive cases, brown as strong positive. SPSS 17.0 software application was used for all analysis and statistical significance was defined as P values less than 0.01.Results:Positive rate of CD8 as T-lymphocytes marker was 83%(50/60), positive rate of CD20 as B-lymphocytes marker was 60%(36/60),47 cases (78%) expressed IL-6 positive,33 cases (55%) had IL-23 and TGF-(3 was 63%(38/60).In the group of CD8, CD20, IL-6, IL-23 expressed positive mostly,the prostate volume were bigger (P<0.01)and the I-PSS scores were higher(P<0.01). The MFR in the group that interstitial cells display TGF-βpositive, was lower than the negeative ones (P<0.01). Conclusion:CD8,CD20,IL-6,IL-23 and TGF-βshowed high expression rates in BPH tissues,also had strong correlation with the clinical data. This point may indicate immune-inflammation play an important role in the development process of BPH and help to complete the pathogenisis theory.