Effects Of Selenium Attenuated Cadmium On Immune Cytokines And Inflammation Cytokines In Chicken Splenic Lymphocytes

Cadmium(Cd) is an environmental pollutant and has potent toxicant to organisms. The extensive use in the industry, has become a major heavy metals of humans and the environment pollution, the main source of pollutants are such as smelting, coal,battery. Selenium(Se) has been known to interact biologically with Cd. Se is a trace mineral in animal and human health has the very important role.This research mainly study Cd/Se in poultry effects on immune factors and inflammatory factors.First of all foster chicken spleen lymphocytes, The study of Cd/Se affect immune and inflammatory cytokines in bird is lacking, and the present study tackled this question partly. The chicken splenic lymphocytes with Cd(10-6 mol/L Cd Cl2), Se(10-7 mol/L Na2 Se O3), Cd+Se(10-7 mol/L Na2 Se O3 and 10-6 mol/L Cd Cl2) and control group were incubated for 12 h, 24 h, 36 h, 48 h, 60 h respectively. At each time point, cells were collected and the m RNA expression levels of IL-1β, IL-2, IL-4, IL-10, IL-17, IFN-γ NF-κB, INOS, COX-2, TNF-α and PGE2 were examined. Compared to the control group and the Se treated group, the mRNA expression level of IL-2, IL-4, IL-10, IL-17 and IFN-γ decreased significantly in the Cd treated group. On the contrary, the m RNA expression level of IL-1β NF-κB, INOS, COX-2, TNF-α and PGE2 increased markedly. In the Cd+Se treated group, IL-2, IL-4, IL-10, IL-17 and IFN-γ were higher than that if in the Cd treated group, but did not reach that of the Se treated groups and the control group. The m RNA expression level of IL-1β NF-κB, INOS, COX-2, TNF-α and PGE2 in the Cd+Se treated group was lower than that of the Cd treated group.The results of protein expression and m RNA expression levels of NF-κB and COX-2 were consistent Se attenuated partly Cd-induced toxicity.