| Objectives: To evaluate the effect of Armillariella tabescens on chemotherapy-induced gastrointestinal tract reaction by cisplatin. To study of the gastric electrical activity and its relationship with 5-HT among Armillariella tabescens for the prevention of chemotherapy-induced gastrointestinal tract reaction by cisplatin.Methods: Forty-eight male Sprague-Dawley rats were randomized into control group, model group, low dose of Armillariella tabescens group, middle dose of Armillariella tabescens group, high dose of Armillariella tabescens group and ondansetron group. Rats were injected intraperitoneally with cisplatin to induce pica. And observe the effect of Armillariella tabescens on consumption of kaolin,food,water and body weight. Ninety male Sprague-Dawley rats were randomized into control group, model group, low, middle and high dose of Armillariella tabescens group, ondansetron group. Rats were injected intraperitoneally with cisplatin 6mg/kg to induce pica according to method of De Jonghe. Using biological function of experimental system to record and analyze electrical activity in smooth muscle of gastric antrum in rat, and Using ELISA technique to assay the concentration of 5-HT in gastrointestinal tract and brain tissue, to observe the effectiveness of Armillariella tabescens to gastric electrical activity and its relationship with 5-HT in different time after replication pica model by DDP.Results: 24-72h after cisplatin administration, in the middle dose of Armillariella tabescens group, the high dose of Armillariella tabescens group and the ondansetron group, kaolin intake lower significantly compared to intake in the model group, respectively (P<0.05). The middle dose of Armillariella tabescens group was significantly higher than the model group in those variables, such as consumption of food during 48-72h (P<0.05), consumption of water during 48-72h (P<0.05), and body weight at 72h (P<0.05), but there were no statistically difference between the ondansetron group and the model group in those variables(P>0.05).24-72h after cisplatin administration, the cycles per minute (CPM) and the amplitude of vibration (AV) of slow-wave on the gastric antrum in the model group were all significantly higher than those in the control group (P<0.05) and the middle, high dose of Armillariella tabescens group (P<0.01), respectively, the most significant difference between the model group (CPM: 7.33±2.92, AV: 249.75±79.09) and the control group (CPM: 3.00±1.55, AV: 148.04±63.51), and the high dose of Armillariella tabescens group (CPM: 4.13±1.14, AV: 163.46±26.14) at 24h after cisplatin administration. 24-72h after cisplatin administration, in the middle and high dose of Armillariella tabescens group, kaolin intake lower significantly compared to intake in the model group, respectively (P<0.05). After cisplatin administration, 5-HT in duodenum and gastric antrum tissue, the middle dose of Armillariella tabescens group at 24h (P<0.01) and the high dose of Armillariella tabescens group during 24-72h (P<0.01) was all significantly lower than the model group, respectively, but 5-HT in pons medulla tissue, only the high dose of Armillariella tabescens group at 24h (P<0.05) was significantly lower than the model group. The mean scores of CMP and AV presented increasing with concentration of 5-HT increased, especially at 24h after cisplatin administration.Conclusions: Armillariella tabescens could effectively inhibit cisplatin-induced pica response, and the middle dose of Armillariella tabescens group were significantly better than the model group in improving the problems of food intake reduction, water intake reduction and body weight loss. Armillariella tabescens might inhibit the release of 5-HT in duodenum and gastric antrum tissue and effectively inhibit the gastric rhythmic movement disorder induced by chemotherapy with DDP in rat, and its mechanisms should be involvement of 5-Hydroxytryptamine.
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