Investigation On The Objective Differences Of Cold And HotProperty In Mahuang Decoction And Maxingshigan Decoction And Their Prescription Compatibilities Based On Thermodynamics

Prescription is a therapeuticsystem with clearly defined law based on the theory of Chinese herbal property and Prescription Compatibility. The theory of Chinese herbal property, in which the Cold and Hot Natural property is the first property of Chinese herb, plays an important role in prescription. Therefore, the prescription compatibility of Cold and Hot natural property is the taproot of Chinese compatibility law. Study on the prescription compatibility of Cold and Hot natural property has a significance both theoretically and practically to the explaining scientific intension of Chinese traditional medicine nature and basic theory of prescription, clinical diagnosis, guiding treatment, normalization of new medicine development and reasonably forming a prescription. Under the guidance of Traditional Chinese Medicine and the Biothermodynamics theory, this paper discusses the objective differences of cold and hot property of MHD and MXSGD from both micro and macro point of view by using the cold/hot plate differentiating technology and microcalorimetry combined with material basis analysis of chemical components, and further explore their complex prescription compatibility law of Cold and Hot property. The main research results are as follows:1. Study of objective differences of cold and hot property of MHD and MXSGD:To study the effects of MHD and MXSGD on normal and Cold/Hot symptomatic mice based on mice temperature tropism by a Cold/Hot plate differentiating technology. The results in the normal mice showed that the remaining ratio on warm pad of MHD group decreased compared with control group, and that of MXSGD increased compared with MHD group. Meanwhile, the objective differences of cold and hot property of MHD and MXSGD were observed between them in both Cold and Hot symptomatic mice. The internal mechanism may be due to energy metabolism and body are closely related to ATPase activity, total antioxidant capacity (T-AOC) and other biochemical indicators change accordingly. MHD and MXSGD in mouse spleen lymphocytes, Escherichia coli and Staphylococcus aureus and other microorganisms are also carriers also showed significant differences between cold and hot property.2. Study of prescription compatibilities of Cold and Hot property of MHD:Compared with MHD, the remaining rate of Consensus Compositions group(CCG), animals on warm pad was found to be significantly increased, revealing a thermotropism, and it also increased in Cinnamomi Ramulus group. In the study of the influence of Cinnamomi Ramulus'dose on the cold&hot property of MHD, Compared with MHD, the remaining rate of MHD with high dose Cinnamomi Ramulus group animals on warm pad was found to be significantly increased. At the same time, Using the microcalorimetry and E. coli, MHD combination effects were discussed, and the results of heat production order showed:MHD> high dose Cinnamomi Ramulus in MHD> CCG> Gui> low-dose Cinnamomi Ramulus in MHD. The results show that the proportion of Cinnamomi Ramulus combined with CCG (MHD) to create synergies and reduce the dose may have Guizhi antagonism.3. Study of prescription compatibilities of Cold and Hot property of MXSGD: MXSGD group, Consensus Compositions Group mice showed "driven cold" enhanced (P<0.05), while showed the "increasingly cold" weakened (P<0.01). Using the Guinness Book of "Q" value method to evaluate the interaction, Consensus Compositions Group in combination with the Gypsum Fibrosum group (MXSGD) showed an antagonistic effect. In the study of Gypsum dose, the results show increasing or decreasing the dose of gypsum does not change the cold and heat properties of MXSGD. However, in the Microcalorimetric study of MXSGD on E. coli, the results showed its heat production order:Consensus Compositions group> low dose of gypsum Maxinshigan soup> MXSGD> high dose of gypsum The MXSGD> gypsum. The results show that with increasing doses of gypsum, MXSGD's "cold property" enhances.4. Study of chemical Material basis:to establish the UPLC fingerprints of MHD and MXSGD. Meanwhile, the content of five main chemical constituents were determined. The results were analyzed by the relative area normalization, similarity analysis and canonical correlation analysis. Both the relative normalized area of the fingerprint and the content from MHD, MXSGD and other drug groups showed that the change of the major chemical compositions were the chemical material base caused transformation between Cold and Hot. And, it was deduced that material basis which affected the transformation between the Cold and Hot was X11,X13, X16, X18 and ephedrine by the canonical correlation analysis and principal component analysis.To sum up, the differences in Cold and Hot properties of MHD and MXSGD might be expressed by the behavior of thermotropism and micro level in an intuitive and objective way using the Cold&Hot plate differentiating technology and microcalorimetry, which verifies the scientific effectiveness of Chinese traditional treatment principle and method of " expelling heat with cold herbs and cryopathy requiring warm prescription".Meanwhile, we have preliminarily determined the chemical materials basis combining the method of chemical component analysis, which might influence the Cold and Hot natural properties of MHD and MXSGD. This research could provide a new visual view angle and experimental reference to the study on the modernization of Cold and Hot Natural Properties theory of Chinese prescription.