The Research Of Anti-inflammatory Effect Mechanism Of Cortex Fraxini For Rats Acute Gouty Arthritis

Objective:From the pathogenesis of gout, establish acute gouty arthritis models discusses the pathogenesis of Cortex Fraxini (TCCF) always coumarin gouty arthritis anti-inflammatory effect mechanism of research, provide ideas and methods for new drugs.Methods:Using Mc Carty DJ classic made mode method,establish acute gouty arthritis model. Observing ankle joint swelling degree rats of change for respectively on made before and after die mould maker different period (2,4,8,12,24,48 and 72 hours). Observed the pathological changes of rat synovial by Hematoxylin—Yihong dyed:1.The infection of white biood cslls (WBC),2. The size of micrangiums. Using enzyme-linked immunosorbent method for detection rat serum Interleukin-1β, interleukin 8 and Tumor necrosis factor-a alpha contentResult:1.TCCF can decline significantiy the swelling of metatarsopalangeal joints. Its inhibition and autumn narcissus alkali quite, but it has high security and with no adverse reaction.2..TCCF can decline significantiy the infection of white biood cslls and the size of micrangiums.3. TCCF can obviously reduce rat serum Interleukin-1β, interleukin 8 and Tumor necrosis factor-a alpha content.4. The role of the TCCF, presents certain concentration-response dependencies Conclusions:TCCF can obviously improve of symptoms for gouty arthritis, it has anti-inflammatory and related with can obviously reduce the infection of white biood cslls and the size of micrangiums.