| Endophytic fungi are living in plants and have no significantly negative effects on the host plants. They have rich diversity of microorganism groups, and they can produce secondary metabolites with biological activity. Therefore, endophytic fungi are concerned as a source of new medicines due to their tremendous potential.In this study we selected Undifilum oxytropis (Q. Wang, Nagao & Kakish) as the research material, and obtained fermented products through the liquid deep fermentation. Then we had preliminary study the extractions, pharmacological activities and chemical constitutions were preliminarily studied.. The results were as follows:1. In this study, ITS1 and ITS4 were selected as primers.The rDNA form Mycelium of Undifilum oxytropis were PCR amplified and the amplified products were sequenced. The result was as followed:The similarity of homologous of Undifilum oxytropis, with Undifilum oxytropis almost reached 99%. Under the microscope, the form of colonies, mycelium, spores of Undifilum oxytropis was Consistent With the preliminary reported. Therefore the bacteria growthed on the new medium was Undifilum oxytropis.2. The chemical constitutions of the fermented products from Embellisia oxytropis were performed as systematic pre-experiment,and the fermented products was preliminarily confirmed that mainly contained Alkaloids, Flavonoids, Anthraquinones, Polysaccharides, Saponins, Essential oil and Phenols and so on. In addition, fermenting liquor contained Polysaccharides, Alkaloids, Flavonoids. Those were similar to the host plants, Oxytropis kansuensis (Leguminosae; Oxytropis DC.). It was showed that endophytic fungi could generate chemical constitutions that were same or similar to the host plants.3. The antioxidant activities of the secondary metabolites of Undifilum oxytropis were investigated by indexes of the total reduction power; inhibition activity of·OH; elimination of O2- and NO2-. The results showed that, in certain concentration range, eliminate ability of polysaccharides from Undifilum oxytropis (intracellular and exocellular) to the total reduction power;·OH; O2- and NO2- increased with the increasing concentrations and showed dose-effect relationship. The antioxidant capacity of intracellular polysaccharide was the best. When the concentration of intracellular polysaccharide was 300μg/ml, the highest light absorption value was 1.594 during the determination of total reducing power. When the concentration was 1.6mg/ml, the eliminate rates for·OH; O2- and NO2- were 86.3%,86.2% and 81.2%, respectively. They were a little lower than those of Vc. The Index of Evaluation in this study were otal reduction power; inhibition activity of·OH; elimination of O2-and NO2-.4. We also preliminarily did the selection studying on the antioxidant activities of each part of ethanol extracts form fermented products by the indexes of the total reduction power; inhibition activity of·OH; elimination of O2- and NO2-. The results showed that, in certain concentration range, the eliminate ability of each polarity part which was extracted by ethanol from secondary metabolites increased with increasing concentrations by the indexes of the total reduction power;·OH; O2-and NO2-. And it also showed a certain antioxidant activities. During the experience the total reduction power; elimination of·OH and NO2-,the ability of mycelia were better than fermenting liquor. When concentration was 1.6mg/ml, the eliminate rate of mycelia chloroform extracts to·OH and NO2- were good, (86.7% and 68.7%, respectively). During the experience on eliminating of O2-, the ability of fermenting liquor was better than mycelia. When concentration was 1.6mg/ml, eliminate rate of fermented n-butanol part was the best, (74.1%).5. We measured the antibacterial activity of ethanol extracts of fermented products from Undifilum oxytropis. The antibacterial experiments used filter paper diffusion method, while selected chloroform, ethyl acetate and n-butyl alcohol parts of ethanol extracts from mycelia and fermenting liquor as test samples, Staphylococcus aureus, Enteritis salmonella, Escherichia coli and Rat typhoid salmonella bacteria were selected as indicator bacteria, and Streptomycin sulfate was selected as positive control. The results showed that, either chloroform extracts of mycelia and fermented liquid or ethyl acetate extracts of mycelia all showed a little antibacterial activity. The antibacterial activities of chloroform extracts from fermenting liquor to Escherichia coli and Staphylococcus aureus were better, the antibacterial diameters were 1.62cm and 1.73cm, the antibacterial rates were 68.2% and 61.4%, respectively. The antibacterial activities of chloroform extracts of mycelia to Staphylococcus aureus, Enteritis salmonella and Rat typhoid salmonella bacteria also were better, the antibacterial rates were 65.4%,59.5% and 60.3%, respectively. The antibacterial activities of ethyl acetate extracts from mycelia was the best, it showed all of the four indicator bacteria have well antibacterial activities.especially on Escherichia coli, the antibacterial rate was 89.6%.6. In this paper, the antitumor activity of polysaccharide and ethanol extract of Undifilum oxytropis was studied. The inhibition rate of high, medium and low dose group of intracellular polysaccharide was 43.91%. 53.12%,35.42%, respectivily, and the inhibition rate of high, medium and low dose group of ethanol extract was 53.87%,48.90%,33.42%, respectivily.7. Chemical constitutions of ethanol and petroleum ether extracts from Embellisia oxytropis were preliminarily investigated through the means of silica gel column chromatography, thin layer chromatography and GC-MS analysis. It showed 22 compounds by GC-MS analysis, and eight kinds of compounds were confirmed which included four alkanes, two olefine acids and two esters.
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