Studies On Chemical Constitudents Of Oxytropis Falcata Bunge And Cleroderdrun Bugei Stued

The chemical constituents and pharmacological activities of oxytropis were summarized in the first part.In this M.D thesis, chemical constituents of Oxytropis falcata Bunge,Cleroderdrun bugei stued had been studied. The compounds were isolated by means of silica gel column chromatography (CC), short column chromatography by reduce pressure and recrystallization et al. The structures of these compounds were elucidated by chemical transformations and spectroscopic identification including IR,EI-MS,FAB-MS,HR-MS,¹HNMR,¹³CNMR,DEPT,¹H-¹H COSY,gHMQC,gHMBC.From the Oxytropis falcata Bunge, the identified sixteen compounds were: N-hydrocinnamoyl-2-phenylethylamine (Y₁), N-benzoyl-phenylethylamine (Y₂), N-cinnamoyl-2-phenylethylamine (Y₃), N-benzoyl-hydroxyphenylethylamine (Y₄), Oxytropine C (Y₅), 3-methoxyaegeline (Y₆), 2'-hydroxy-4'-methoxychalcone (Y₇), 7-methoxyflavanone (Y₈), 5-hydroxy-7-methoxyflavanone (Y₉), rhamnetin (Y₁₀), oxytriphine (Y₁₁), anagyrine (Y₁₂), lupanine (Y₁₃),Kaempfero3-O-(6"-acetyl)-β-D-glucoside (Y₁₄), 2-monolinoleoyin (Y₁₅),β-sitosterol (Y₁₆). Compounds 1 and 5 have not been reported previously, and compounds 6-9 were obtained from this plant for the frist time. From the Cleroderdrun bugei stued, the identifyied four compounds were: pruning (1), nairutin (2), dyaimid (3), apigenin(4).Furthermore, the bioactivities of some compounds were studied using SRB method to evaluate their cytotoxic activity against SGC, Compounds Y₇ and Y₈ showed appreciable cytotoxic activity against these cancer cell. The results of this research can provide some scientific evidence for the selectivity and development of traditional Chinese medicine.