The Protectional Roles And Significance Of Baicalin Zinc On Immunological Liver Injury In Rats

Objective:To build model of immunological liver injury in rats, we use BCG plus LPS The experimental group given therapeutic doses of Baicalin Zinc by gastric irrigation, and then we observed the general situiation,biochemical parameters and maturity process. To provide theoretical support and more choice for clinical medication, we study the protectional roles, mechanisms and significance of Baicalin Zinc on immunological liver injury in rats.Methods:1,the 70 rats were randomly divided into blank control group A(10 cases), baicalin group B (15 cases), Baicalin Zinc group C (15 cases), Reduced Glutathione Sodium group D (15 cases) and model group E (15 cases).2,To build model of immunological liver injury in rats, we use BCG plus LPS. First, escept the blank control group, all the other groups were sensitized by given BCG, meanwhile, given the appropriate drugs, but the blank control group A and model group E were given CMC-Na by gastric irrigation. All the groups, except the blank control group, were given LPS by intraperitoneal injection at the 11th day. After 24 hours, we tested the GSH-Px of the hepatic tissue, ALT and AST. At the same time, we obversed the pathological sections which were made of the right lobe of liver tissue, and the liver tissue were studied on by immunohistochemictry.3,Then, all the groups were given the appropriate drugs. The drug given groups and the model group were divided into group 1 and group 2. The rats in the group 1 were given LPS again; the group 2 were given the appropriate drugs contiuntly.10 days latter, we tested the GSH-Px of the hepatic tissue, ALT and AST., meanwhile, we obversed the pathological sections which were made of the right lobe of liver tissue, and the liver tissue were studied on by immunohistochemictry. Excepting blank control group A and model group E, other groups continued to give the appropriate drugs,10 days as a cycle. At last,we did all the tests as usual.All the datas were anaiyized by SPSS software.Result:The immunological liver injury model was builded sucessfully.24 hours after injected LPS at the 11th days, the ALT and AST of the model group were both more than the blank, but the GSH-Px is less. The difference had statistical significance(P<0.05). The three drug given groups were compared with the model group one by one, the results showed that the difference in AST and ALT had statistical significance(P<0.05), though the GSH-Px is more than the model group, there is no statistical significance between them. The difference between the three drug given groups had no statistical significance. And then compared with the 11th days, at the 20 days, the group 2 of the drug given groups were given the drugs, but no LPS. The numbers of ALT, AST and GSH-Px in Baicalin Zinc group and Reduced Glutathione Sodium group is more different.The numbers of the 20 days, there were statistical significance between group 2 of the Baicalin Zinc group C and baicalin group, group 2 of the Baicalin Zinc group and the model, group 2 of the Baicalin Zinc group and the blank((P<0.05); the GSH-Px is different in the last two above. At the 30 days, the differents of AST, ALT and GSH-Px between the group 1 of the drug given and the model groups and the blank had statistical significance, but the number between the group 1 of the Baicalin Zinc group and the group 1 of the Reduced Glutathione Sodium group were no sigificant different. Observeing the pathological sections, the inflammatory injury of the liver in the drug given groups is weakless than the model group, and the quantity of the CYP2E1 is a little higher than the model group.Conclusion:The rats were dealed with BCG and appropriate drugs, and then given LPS, which induced the immunological liver injury were found. The injury of the liver is less than the model group.Baicalin Zinc can improve the antioxidant capacity, and reduce the cytotoxicity of the immune complexes,so it can reduce the injury of the liver. At the same time, the antioxidant capacity of the Baicalin Zinc is more than the role of baicalin, and as same as the Reduced Glutathione Sodium. The Baicalin Zinc is another choice of drugs which is usfull of protecting liver in clinical.