| Chemiluminescence analysis has become an important apartment of trace analysis, which has been widely applied in pharmaceutical and life science. Chemiluminescence analysis needs no light source to avoid the effects of stray light and the instability of light source. Therefore, it has excellent sensitivity. Combined with flow-injection analysis, the reproducibility and selectivity of the method have been enhanced. And the automation of the method is realized, which makes the on-line determination come true. This combination expanded the application of this method in quantitative analysis. During the continue exploring of chemiluminescence, people discovered that when some new substances were put into the mixture which the reaction has finished, a new chemiluminescence would appear. This phenomenon was call second-chemiluminescence. It was proofed to be a universal phenomenon, which existed in many systems and many substances have been determined. The discovery of second-chemiluminescence was important to enrich and expand its research contents, deepen the comprehension of chemiluminescence, thus, to be applied into determination effectively.Chapter 1:SummarizatonThe mechanism and principles of chemiluminescence analysis, the common chemiluminescence systems, the combination of chemiluminescence with other techniques, the researches and applications of chemiluminescence analysis in pharmaceutical and life science in recent years, second-chemiluminescence and its common systems have been summarized. The purpose of this research was put forward.Chapter 2:Determination of epinephrine based on luminol-potassium periodate second-chemiluminescence systemA second chemiluminescence (SCL) phenomenon was observed when epinephrine was injected into the mixture of luminol and potassium periodate solution after their chemiluminescence (CL) reaction reacted adequately. The possible mechanism is propounded with the studies of the CL kinetic characteristics, the CL spectra. Based on the SCL reaction, a rapid and sensitive method for the determination of epinephrine was established, in which stopped flow technique was used. The linear response range was from 3.0×10-5 g/L to 1.2×10-4 g/L with a linear correlation coefficient of 0.9969. The relative standard deviation for 5.0×10-5 g/L epinephrine was 2.0%(n=11). The detection limit was 3.0×10-1g/L. The method has been applied to the determination of epinephrine in pharmaceutical samples with satisfactory results.Chapter 3:Second-chemiluminescence determination of chloramphenicol based on luminol-potassium periodate systemA second-chemiluminescence phenomenon was observed when chloramphenicol was injected into the mixture of luminol and potassium periodate after the chemiluminescence reaction of luminol-potassium periodate had finished. The possible reaction mechanism was proposed based on the studies of the CL kinetic characteristics, the CL spectra, the fluorescence spectra, and the UV absorption spectra of the related substances. Based on the SCL reaction, a rapid and sensitive method for the determination of chloramphenicol was established. The linear response range was from 6.0 x 10-7 mol/L to 1.0×10-5 mol/L with a correlation coefficient of 0.9986. The relative standard deviation for 5.0×10-6 mol/L chloramphenicol was 2.3%(n=11). The detection limit was 1.6 10-7 mol/L. The method has been applied to the determination of chloramphenicol in pharmaceutical samples with satisfactory results.Chapter 4:Second-chemiluminescence determination of neomycin based on luminol-potassium periodate systemA second-chemiluminescence phenomenon was observed when neomycin was injected into the mixture of luminol and potassium periodate after the chemiluminescence reaction of luminol-potassium periodate had finished. Based on the second-chemiluminescence reaction, a rapid and sensitive method for the determination of neomycin was established. The linear response range was from 2.0×10 tol.4×10 g/L. The relative standard deviation for 5.0×10 g/L neomycin was 2.5%(n=9). The detection limit was 6.7×10-4 g/L. The method has been applied to the determination of neomycin in pharmaceutical samples with satisfactory results.Chapter 5:Chemiluminescence determination of ferulic acid by flow-injection analysis using potassium permanganate sensitized by rhodamine 6GA sensitive, rapid and simple flow-injection chemiluminescence method has been developed for the determination of ferulic acid based on the chemiluminescence reaction of ferulic acid with rhodamine 6G and potassium permanganate in sulphuric acid medium. Chemiluminescence signal was observed when ferulic acid was injected into the mixture of acidic potassium permanganate solution and rhodamine 6G solution in a flow-cell. The present method allowed the determination of ferulic acid in the concentration range of 8.0×10-7 to 1.0×10-5 mol/L and the detection limit for ferulic acid was 3.3×10-8 mol/L. The relative standard deviation was 1.1% for 11 replicate analyses of 1.0×105 mol/L ferulic acid. The proposed method was applied to the determination of ferulic acid in pharmaceutical preparation with satisfactory results.
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