Application Of Flow-injection Chemiluminescence In The Quinolone Kinds Of Pharmaceutical Analysis

Chemiluminescence(CL) analysis offers the advantages of high sensitivity , wide linerly dynamic range and simple instruments. Especially coupled with flow-injection analysis (FIA), the CL methods provide cheap, rapid, simple and reproducible means of detection. However, the relatively poor selectivity of the CL method itself limits its direct application to the analysis of analyte in complicated samples. The common way to solve the problem is that the CL method was applied combination with some separation techniques such as high-performance liquid chromatography, low-pressure ion chromatography, capillary electrophoresis, molecular imprinting and solid extracton. Therefore, the CL analysis methods have aready become an essential tool in many fields such as pharmaceutical analysis, environmental science and biological engineering.Quinlones is the new generation broad-spectrum antibities developed quickly and is widely used for clinic in recent years. It has the characteristic of broad-spectrum antibiotics, simple structures, small side-effect and cross-resistance with other antibities drugs. This synthetic fluoroquinolone derivative is widespread used in the treatment of infections of urinary tract, respiratory tract, gastroenteritis, skin and skin structures infections. So it is one of the most active fields to the exploitation of anti-infected sites. But the problems were causeded by the abuse of this kind of pharmaceutical is strictly. The results of pharmaceutical was abused not only contaminated the environment but also increase the resistence, even endenger the life of human beings. Thus, it is necessary to studied and founded the quick and sensitive analysis methods of quinlones and it is also impotant to the new drugs exploitation, pharmacological studying and the clinic application. This thesis penetrates the sensitive CL methods for quinolones, studies the CL reaction of levofloxacin, lomefloxacin and gatifloxacin. The methods have been applied to assay the samples and satisfactory results were obtained. In the end we discussed the CL mechanism of these systems. It includes four chapters as follows:...